Kazan Federal University Digital Repository

LC-MS Method Development for Simultaneous Determination of Trans-3′-hydroxycotinine and Three Mercapturic Acids in Urine

Show simple item record

dc.contributor.author Lopukhov L.
dc.contributor.author Laikov A.
dc.contributor.author Romanova V.
dc.contributor.author Gatina D.
dc.contributor.author Lopukhov V.
dc.contributor.author Abdulkhakov S.
dc.contributor.author Zaitseva T.
dc.contributor.author Medvedeva S.
dc.contributor.author Gnuchikh E.
dc.contributor.author Salafutdinov I.
dc.contributor.author Grigoryeva T.
dc.date.accessioned 2019-01-22T20:57:28Z
dc.date.available 2019-01-22T20:57:28Z
dc.date.issued 2018
dc.identifier.issn 2191-1630
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/149608
dc.description.abstract © 2018, Springer Science+Business Media, LLC, part of Springer Nature. The negative impact of tobacco smoke on the human body is due to a wide range of harmful substances including volatile organic compounds (VOCs). Some VOCs of tobacco smoke metabolize in human organism into mercapturic acids (MAs). The determination of the amount of MAs in readily available biological fluids, for example in urine, allows to assess the level of exposure of these VOCs in a particular person. It is useful to assess the impact of individual VOCs on the body together with the assessment of the intake of nicotine. The intake of nicotine can be determined by the content of its metabolites in the urine, in particular by the content of trans-3′-hydroxycotinine (tH-Cot). A joint assessment of the concentrations of trans-3′-hydroxycotinine and MAs in urine allows obtaining selective information about effects of different VOCs and nicotine on the smoker’s body. We have developed a liquid chromatography–mass spectrometry (LC-MS) method for simultaneous quantifying of tH-Cot and three MAs: N-Acetyl-S-(3-hydroxypropyl)cysteine (HPMA), N-Acetyl-S-(3-hydroxypropyl-1-methyl)-L-cysteine (HMPMA), N-Acetyl-S-(2-cyanoethyl)-L-cysteine (CEMA). We used this method to quantify the levels of MAs and tH-Cot in the urine of a group of 15 smokers just before and 5 days after smoking cessation. For all studied compounds, we have found statistically significant changes in concentration on the fifth day of smoking cessation. The method developed can be used to jointly assess the levels of exposure to nicotine and VOCs in the study of various tobacco products.
dc.relation.ispartofseries BioNanoScience
dc.subject LC-MS
dc.subject Mercapturic acids
dc.subject Nicotine
dc.subject Tobacco
dc.title LC-MS Method Development for Simultaneous Determination of Trans-3′-hydroxycotinine and Three Mercapturic Acids in Urine
dc.type Article
dc.relation.ispartofseries-issue 3
dc.relation.ispartofseries-volume 8
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 924
dc.source.id SCOPUS21911630-2018-8-3-SID85052687923

Files in this item

This item appears in the following Collection(s)

  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

Show simple item record

Search DSpace

Advanced Search


My Account