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Extraction and Serological Properties of Mycobacterium Cell Surface and Excreted Proteins

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dc.contributor.author Akhmadeev R.
dc.contributor.author Samigullina E.
dc.contributor.author Nabatov A.
dc.contributor.author Shuralev E.
dc.date.accessioned 2019-01-22T20:57:19Z
dc.date.available 2019-01-22T20:57:19Z
dc.date.issued 2018
dc.identifier.issn 2191-1630
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/149595
dc.description.abstract © 2017, Springer Science+Business Media, LLC, part of Springer Nature. Modern medicine still faces the task of distinguishing active and latent tuberculosis cases at the early stage of the disease. Serological approaches have their advantages for their use in diagnostics. However, the progress of these approaches is ongoing but further progress is needed to meet the needs for this disease. Here, we extracted Mycobacterium tuberculosis H37Rv proteins from culture medium or from the cell surface and studied their reactivity with anti-M. tuberculosis serum in both ELISA and immunoblots. We found that M. tuberculosis surface proteins, extracted using dimethyl sulfoxide, with molecular weights in the range of 6.5–200 kDa, showed strong specific reactivity with anti-M. tuberculosis positive serum. While excreted proteins in the molecular weight range of 32–45 kDa had the highest reactivity. The latter was confirmed serologically when very weak signal was detected from the filtrate fractions compared to stronger activity from the Vivaspin 50 kDa MWCO retentates. Moreover, Mycobacterium bovis and tuberculosis proteins from the filter retentates had clear specific serum reactivity, which suggests that this approach can be used for differential diagnosis of two infections.
dc.relation.ispartofseries BioNanoScience
dc.subject Antigens
dc.subject DMSO
dc.subject Immunoblot
dc.subject Mycobacterium tuberculosis
dc.subject Proteins
dc.subject Vivaspin
dc.title Extraction and Serological Properties of Mycobacterium Cell Surface and Excreted Proteins
dc.type Article
dc.relation.ispartofseries-issue 1
dc.relation.ispartofseries-volume 8
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 459
dc.source.id SCOPUS21911630-2018-8-1-SID85044383044


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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