The Effect of Mammalian Cell Functionalization with Polycation and Halloysite Nanotubes on Intercellular Interactions

Abstract

© 2017, Springer Science+Business Media, LLC. This paper describes the formation of a cytoprotective frame on mammalian cells and determines its impact on cell viability and creation of 3D tissue-like structures from cells modified by halloysite nanotubes and a polycation. Using the “hanging drop” method, spheroids were obtained from unmodified cells and cells modified with halloysite nanotubes and poly(acrylamide-co-diallyldimethyl-ammonium chloride). It has been shown that layer-by-layer modification of eukaryotic cells with the natural mineral halloysite and the polymer has little effect on their physiological parameters. Probably, the functionalization of the surface of cells by the nanoframe can be used for their protection from lethal factors, as well as for targeted drug delivery.