Fourier Transform Infrared Spectroscopy Analysis of Human Osteosarcoma Bone Tissue

Abstract

© 2018, Springer Science+Business Media, LLC, part of Springer Nature. Since the middle of the XXth century, Fourier transform infrared (FT-IR) spectroscopy has been employed as a nondestructive, label-free, highly sensitive, and specific analytical method with many potential applications in different fields of biomedical research and in particular cancer research and diagnosis. In this study, infrared spectra of normal human bone and tumor tissue of osteosarcoma were analyzed using FT-IR spectroscopy in the range of 800–1800 cm−1. The results revealed that some of the spectral characteristics varied significantly between normal and malignant tissues, that is, IR peak positions, and the spectral intensities. The main changes in the spectral features of tissues were observed in the phosphate ν1, ν3 contour (950–1200 cm−1) and Amide I (1620–1680 cm−1) and II (1520–1570 cm−1) bands. The molecular interpretation of the differences between normal and pathological states of bone tissues may indicate the changes in hydroxyapatite mineralization and collagen structure of the malignant tissue. The most important application of this technique is evaluation of the disease states and the results of therapeutic intervention under the medical treatment.