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QM/MM description of newly selected catalytic bioscavengers against organophosphorus compounds revealed reactivation stimulus mediated by histidine residue in the acyl-binding loop

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dc.contributor.author Zlobin A.
dc.contributor.author Mokrushina Y.
dc.contributor.author Terekhov S.
dc.contributor.author Zalevsky A.
dc.contributor.author Bobik T.
dc.contributor.author Stepanova A.
dc.contributor.author Aliseychik M.
dc.contributor.author Kartseva O.
dc.contributor.author Panteleev S.
dc.contributor.author Golovin A.
dc.contributor.author Belogurov A.
dc.contributor.author Gabibov A.
dc.contributor.author Smirnov I.
dc.date.accessioned 2019-01-22T20:55:32Z
dc.date.available 2019-01-22T20:55:32Z
dc.date.issued 2018
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/149446
dc.description.abstract © 2018 Zlobin, Mokrushina, Terekhov, Zalevsky, Bobik, Stepanova, Aliseychik, Kartseva, Panteleev, Golovin, Belogurov, Gabibov and Smirnov. Butyrylcholinesterase (BChE) is considered as an efficient stoichiometric antidote against organophosphorus (OP) poisons. Recently we utilized combination of calculations and ultrahigh-throughput screening (uHTS) to select BChE variants capable of catalytic destruction of OP pesticide paraoxon. The purpose of this study was to elucidate the molecular mechanism underlying enzymatic hydrolysis of paraoxon by BChE variants using hybrid quantum mechanical/molecular mechanical (QM/MM) calculations. Detailed analysis of accomplished QM/MM runs revealed that histidine residues introduced into the acyl-binding loop are always located in close proximity with aspartate residue at position 70. Histidine residue acts as general base thus leading to attacking water molecule activation and subsequent SN2 inline hydrolysis resulting in BChE reactivation. This combination resembles canonical catalytic triad found in active centers of various proteases. Carboxyl group activates histidine residue by altering its pKa, which in turn promotes the activation of water molecule in terms of its nucleophilicity. Observed re-protonation of catalytic serine residue at position 198 from histidine residue at position 438 recovers initial configuration of the enzyme's active center, facilitating next catalytic cycle. We therefore suggest that utilization of uHTS platform in combination with deciphering of molecular mechanisms by QM/MM calculations may significantly improve our knowledge of enzyme function, propose new strategies for enzyme design and open new horizons in generation of catalytic bioscavengers against OP poisons.
dc.subject Bioscavenger
dc.subject Butyrylcholinesterase
dc.subject Computer design
dc.subject Organophosphorus compound
dc.subject Paraoxon
dc.subject Ultrahigh-throughput screening
dc.title QM/MM description of newly selected catalytic bioscavengers against organophosphorus compounds revealed reactivation stimulus mediated by histidine residue in the acyl-binding loop
dc.type Article
dc.relation.ispartofseries-issue AUG
dc.relation.ispartofseries-volume 9
dc.collection Публикации сотрудников КФУ
dc.source.id SCOPUS-2018-9--SID85054930271


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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