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Diagnostics of autoimmune neurodegeneration using fluorescent probing

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dc.contributor.author Lomakin Y.
dc.contributor.author Kudriaeva A.
dc.contributor.author Kostin N.
dc.contributor.author Terekhov S.
dc.contributor.author Kaminskaya A.
dc.contributor.author Chernov A.
dc.contributor.author Zakharova M.
dc.contributor.author Ivanova M.
dc.contributor.author Simaniv T.
dc.contributor.author Telegin G.
dc.contributor.author Gabibov A.
dc.contributor.author Belogurov A.
dc.date.accessioned 2019-01-22T20:54:43Z
dc.date.available 2019-01-22T20:54:43Z
dc.date.issued 2018
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/149382
dc.description.abstract © 2018, The Author(s). The discovery of antibody-mediated catalysis was a breakthrough that showed antibody function is not limited to specific binding interactions, and that immunoglobulins (Igs) may also chemically transform their target antigens. Recently, so-called “natural catalytic antibodies” have been intimately linked with several pathologies, where they either protect the organism or contribute to the development of autoimmune abnormalities. Previously, we showed that myelin-reactive autoantibodies from patients with multiple sclerosis (MS) and mice with experimental autoimmune encephalomyelitis (EAE) exhibit the ability to recognize and hydrolyse distinct epitopes within myelin basic protein (MBP). Further, the antibody-mediated cleavage of encephalitogenic MBP peptide 81–103, flanked by two fluorescent proteins, can serve as a novel biomarker for MS. Here, we report the next generation of this biomarker, based on the antibody-mediated degradation of a novel chemically synthesized FRET substrate, comprising the fluorophore Cy5 and the quencher QXL680, interconnected by the MBP peptide 81–99: Cy5-MBP81–99-QXL680. This substrate is degraded upon incubation with either purified antibodies from MS patients but not healthy donors or purified antibodies and splenocytes from EAE but not from non-immunized mice. Data presented herein suggest the elaboration of potential specific, rapid, and sensitive diagnostic criteria of active progressive MS.
dc.title Diagnostics of autoimmune neurodegeneration using fluorescent probing
dc.type Article
dc.relation.ispartofseries-issue 1
dc.relation.ispartofseries-volume 8
dc.collection Публикации сотрудников КФУ
dc.source.id SCOPUS-2018-8-1-SID85052237734

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  • Публикации сотрудников КФУ Scopus [22633]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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