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dc.contributor.author | Pashirova T. | |
dc.contributor.author | Zueva I. | |
dc.contributor.author | Petrov K. | |
dc.contributor.author | Lukashenko S. | |
dc.contributor.author | Nizameev I. | |
dc.contributor.author | Kulik N. | |
dc.contributor.author | Voloshina A. | |
dc.contributor.author | Almasy L. | |
dc.contributor.author | Kadirov M. | |
dc.contributor.author | Masson P. | |
dc.contributor.author | Souto E. | |
dc.contributor.author | Zakharova L. | |
dc.contributor.author | Sinyashin O. | |
dc.date.accessioned | 2019-01-22T20:38:37Z | |
dc.date.available | 2019-01-22T20:38:37Z | |
dc.date.issued | 2018 | |
dc.identifier.issn | 0927-7765 | |
dc.identifier.uri | https://dspace.kpfu.ru/xmlui/handle/net/148091 | |
dc.description.abstract | © 2018 Elsevier B.V. New mixed cationic liposomes based on L-α-phosphatidylcholine and dihexadecylmethylhydroxyethylammonium bromide (DHDHAB) were designed to overcome the BBB crossing by using the intranasal route. Synthesis and self-assembly of DHDHAB were performed. A low critical association concentration (0.01 mM), good solubilization properties toward hydrophobic dye Orange OT and antimicrobial activity against gram-positive bacteria Staphylococcus aureus (MIC=7.8 μg mL–1) and Bacillus cereus (MIC=7.8 μg mL–1), low hemolytic activities against human red blood cells (less than 10%) were achieved. Conditions for preparation of cationic vesicles and mixed liposomes with excellent colloidal stability at room temperature were determined. The intranasal administration of rhodamine B-loaded cationic liposomes was shown to increase bioavailability into the brain in comparison to the intravenous injection. The cholinesterase reactivator, 2-PAM, was used as model drug for the loading in cationic liposomes. 2-PAM-loaded cationic liposomes displayed high encapsulation efficiency (∼ 90%) and hydrodynamic diameter close to 100 nm. Intranasally administered 2-PAM-loaded cationic liposomes were effective against paraoxon-induced acetylcholinesterase inhibition in the brain. 2-PAM−loaded liposomes reactivated 12 ± 1% of brain acetylcholinesterase. This promising result opens the possibility to use marketed positively charged oximes in medical countermeasures against organophosphorus poisoning for reactivation of central acetylcholinesterase by implementing a non-invasive approach, via the “nose-brain” pathway. | |
dc.relation.ispartofseries | Colloids and Surfaces B: Biointerfaces | |
dc.subject | Acetylcholinesterase | |
dc.subject | Cationic liposomes | |
dc.subject | Drug delivery systems | |
dc.subject | Organophosphorus agent | |
dc.subject | Paraoxon | |
dc.subject | Pralidoxime chloride | |
dc.subject | Self-assembly | |
dc.subject | Surfactant | |
dc.title | Mixed cationic liposomes for brain delivery of drugs by the intranasal route: The acetylcholinesterase reactivator 2-PAM as encapsulated drug model | |
dc.type | Article | |
dc.relation.ispartofseries-volume | 171 | |
dc.collection | Публикации сотрудников КФУ | |
dc.relation.startpage | 358 | |
dc.source.id | SCOPUS09277765-2018-171-SID85050484821 |