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Evidence of oligomerization of bovine insulin in solution given by NMR

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dc.contributor.author Efimov S.
dc.contributor.author Zgadzay Y.
dc.contributor.author Tarasova N.
dc.contributor.author Klochkov V.
dc.date.accessioned 2019-01-22T20:35:48Z
dc.date.available 2019-01-22T20:35:48Z
dc.date.issued 2018
dc.identifier.issn 0175-7571
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/147887
dc.description.abstract © 2018, European Biophysical Societies' Association. The protein hormone insulin exists in several forms in nature, and a large number of modified sequences are used in pharmacy. They differ by physicochemical properties and efficiency of biological action. Pancreatic bovine insulin was studied in an acidic solution by nuclear magnetic resonance spectroscopy. 1H and 13C NMR signal assignment of backbone and side chains was made by analysis of a set of 2D spectra obtained on a sample with natural isotope abundance. The presence of certain secondary structure elements was revealed on a qualitative level based on nuclear Overhauser effect spectroscopy, which are similar to those observed in the crystal structure. The C-terminus of the B-chain possessed a remarkable flexibility. The molecule was shown to exist in exchange with oligomers based on its self-diffusion coefficient and correlation time measurements performed at different concentrations. Certain signals in the NOESY and HSQC spectra are consistent with the presence of minor conformers; this is an obstacle in simulating the molecular structure under the conditions used in the experiment.
dc.relation.ispartofseries European Biophysics Journal
dc.subject Bovine insulin
dc.subject DOSY
dc.subject NMR
dc.subject NOESY
dc.subject Oligomerization
dc.subject Secondary structure
dc.title Evidence of oligomerization of bovine insulin in solution given by NMR
dc.type Article
dc.relation.ispartofseries-issue 8
dc.relation.ispartofseries-volume 47
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 881
dc.source.id SCOPUS01757571-2018-47-8-SID85047950140


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  • Публикации сотрудников КФУ Scopus [22633]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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