Kazan Federal University Digital Repository

EPR Detection of DNA Interaction with 3-Carboxy-proxyl-Labelled Recombinant Human Histone H1.3

Show simple item record

dc.contributor.author Chasov V.
dc.contributor.author Rodionov A.
dc.contributor.author Gafurov M.
dc.contributor.author Kamalov M.
dc.contributor.author Abdullin T.
dc.contributor.author Zaitsev S.
dc.contributor.author Rizvanov A.
dc.date.accessioned 2018-09-19T23:13:03Z
dc.date.available 2018-09-19T23:13:03Z
dc.date.issued 2017
dc.identifier.issn 2191-1630
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/146084
dc.description.abstract © 2016, Springer Science+Business Media New York.Nitroxide spin labelling is exploited in electron paramagnetic resonance (EPR) spectroscopy to study biological interactions and analysis of conformational changes in protein structures. In our study, recombinant human histone H1.3 was modified by 3-carboxy-proxyl using carbodiimide/N-hydroxysuccinimide coupling. Resulting conjugate of histone H1.3 with the spin label preserved its DNA binding capacity and formed complexes with plasmid DNA. The changes in EPR signal of the spin label were revealed upon conjugate interaction with plasmid DNA. Our preliminary data show the possibility of DNA sensing with nitroxide spin labelled histone H1.3 by EPR spectroscopy.
dc.relation.ispartofseries BioNanoScience
dc.subject Biosensing
dc.subject Electron paramagnetic resonance
dc.subject Nitroxide spin label
dc.subject Plasmid DNA
dc.subject Recombinant human histone H1.3
dc.title EPR Detection of DNA Interaction with 3-Carboxy-proxyl-Labelled Recombinant Human Histone H1.3
dc.type Article
dc.relation.ispartofseries-issue 1
dc.relation.ispartofseries-volume 7
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 109
dc.source.id SCOPUS21911630-2017-7-1-SID85017514062


Files in this item

This item appears in the following Collection(s)

  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

Show simple item record

Search DSpace


Advanced Search

Browse

My Account

Statistics