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dc.contributor.author | Sokurenko Y. | |
dc.contributor.author | Kolpakov A. | |
dc.contributor.author | Ilinskaya O. | |
dc.date.accessioned | 2018-09-19T23:12:52Z | |
dc.date.available | 2018-09-19T23:12:52Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 2191-1630 | |
dc.identifier.uri | https://dspace.kpfu.ru/xmlui/handle/net/146078 | |
dc.description.abstract | © 2016, Springer Science+Business Media New York.Binase, the ribonuclease secreted by Bacillus pumilus, is an endonuclease that cleaves the phosphodiester bond between the 3′-guanyl residue and 5′-OH residue of an adjacent nucleotide, with the formation of a corresponding intermediate, 2′,3′-cGMP on the first stage of a catalytic reaction. Binase possesses selective antitumor effect and induces apoptosis of lung carcinoma A549 cells. It was shown that the 2′,3′-cGMP messenger could exist in the reaction mixture over an hour. Furthermore, the addition of divalent non-transition metal Mg2+ increases the level of 2′,3′-cGMP formed by binase and that could be associated with the stabilization of RNA tertiary structure by this metal. It has been shown that exogenous 2′,3′-cGMP does not induce apoptosis of A549 cells, which are sensitive to binase. However, taking into account the cell-penetrating ability of binase, it can be concluded that 2′,3′-cGMP contributes to apoptogenic binase action only when it is formed intracellularly. | |
dc.relation.ispartofseries | BioNanoScience | |
dc.subject | 2′,3′-cGMP | |
dc.subject | A549 | |
dc.subject | Binase | |
dc.subject | Cycle-forming guanyl-preferring RNases | |
dc.subject | Human lung carcinoma | |
dc.subject | Mg 2+ | |
dc.subject | RNA structure stabilization | |
dc.title | Mg<sup>2+</sup> Enhances the Formation of 2′,3′-cGMP, an Intermediate of RNA Cleavage by Binase | |
dc.type | Article | |
dc.relation.ispartofseries-issue | 1 | |
dc.relation.ispartofseries-volume | 7 | |
dc.collection | Публикации сотрудников КФУ | |
dc.relation.startpage | 167 | |
dc.source.id | SCOPUS21911630-2017-7-1-SID85017506751 |