Kazan Federal University Digital Repository

Mg<sup>2+</sup> Enhances the Formation of 2′,3′-cGMP, an Intermediate of RNA Cleavage by Binase

Show simple item record

dc.contributor.author Sokurenko Y.
dc.contributor.author Kolpakov A.
dc.contributor.author Ilinskaya O.
dc.date.accessioned 2018-09-19T23:12:52Z
dc.date.available 2018-09-19T23:12:52Z
dc.date.issued 2017
dc.identifier.issn 2191-1630
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/146078
dc.description.abstract © 2016, Springer Science+Business Media New York.Binase, the ribonuclease secreted by Bacillus pumilus, is an endonuclease that cleaves the phosphodiester bond between the 3′-guanyl residue and 5′-OH residue of an adjacent nucleotide, with the formation of a corresponding intermediate, 2′,3′-cGMP on the first stage of a catalytic reaction. Binase possesses selective antitumor effect and induces apoptosis of lung carcinoma A549 cells. It was shown that the 2′,3′-cGMP messenger could exist in the reaction mixture over an hour. Furthermore, the addition of divalent non-transition metal Mg2+ increases the level of 2′,3′-cGMP formed by binase and that could be associated with the stabilization of RNA tertiary structure by this metal. It has been shown that exogenous 2′,3′-cGMP does not induce apoptosis of A549 cells, which are sensitive to binase. However, taking into account the cell-penetrating ability of binase, it can be concluded that 2′,3′-cGMP contributes to apoptogenic binase action only when it is formed intracellularly.
dc.relation.ispartofseries BioNanoScience
dc.subject 2′,3′-cGMP
dc.subject A549
dc.subject Binase
dc.subject Cycle-forming guanyl-preferring RNases
dc.subject Human lung carcinoma
dc.subject Mg 2+
dc.subject RNA structure stabilization
dc.title Mg<sup>2+</sup> Enhances the Formation of 2′,3′-cGMP, an Intermediate of RNA Cleavage by Binase
dc.type Article
dc.relation.ispartofseries-issue 1
dc.relation.ispartofseries-volume 7
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 167
dc.source.id SCOPUS21911630-2017-7-1-SID85017506751


Files in this item

This item appears in the following Collection(s)

  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

Show simple item record

Search DSpace


Advanced Search

Browse

My Account

Statistics