dc.contributor.author |
Zakharov A. |
|
dc.contributor.author |
Koroleva K. |
|
dc.contributor.author |
Giniatullin R. |
|
dc.date.accessioned |
2018-09-19T23:12:18Z |
|
dc.date.available |
2018-09-19T23:12:18Z |
|
dc.date.issued |
2016 |
|
dc.identifier.issn |
2191-1630 |
|
dc.identifier.uri |
https://dspace.kpfu.ru/xmlui/handle/net/146060 |
|
dc.description.abstract |
© 2016, Springer Science+Business Media New York.We previously reported the pronociceptive effect of extracellular ATP on meningeal branches of the trigeminal nerve. Based on this, we proposed ATP-driven purinergic mechanisms as important contributors to migraine pain. However, the neurochemical profile of the single nociceptive fibers composing the trigeminal nerve remained unsolved. Here, we present a clustering approach to separate and characterize ATP-induced nociceptive spikes obtained from the trigeminal nerve using a suction electrode. By combining a number of procedures to ensure adequate separation of spikes into clearly distinguishable clusters, we were able to reconstruct single fiber activity, which was characterized by a uniformity of spike shapes and specific signatures of the autocorrelation function. This allows us to reveal, at single fiber level, the sustained time-course of responses to ATP and their respective firing frequencies. These firing frequencies predicted the temporal summation of pain signals in the brainstem and spinal cord. Our findings contribute to a better understanding of the peripheral purinergic mechanisms of trigeminal pain, including migraine pain, and suggest a novel reliable approach for testing other pronociceptive agents potently implicated in migraine. |
|
dc.relation.ispartofseries |
BioNanoScience |
|
dc.subject |
ATP |
|
dc.subject |
Cluster analysis |
|
dc.subject |
Migraine |
|
dc.subject |
Pain |
|
dc.subject |
Patch clamp |
|
dc.subject |
Trigeminal neuron |
|
dc.title |
Clustering Analysis for Sorting ATP-Induced Nociceptive Firing in rat Meninges |
|
dc.type |
Article |
|
dc.relation.ispartofseries-issue |
4 |
|
dc.relation.ispartofseries-volume |
6 |
|
dc.collection |
Публикации сотрудников КФУ |
|
dc.relation.startpage |
508 |
|
dc.source.id |
SCOPUS21911630-2016-6-4-SID84999861841 |
|