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Inactivation of Chromosomal Genes in Serratia marcescens

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dc.contributor.author Kamaletdinova L.
dc.contributor.author Nizamutdinova E.
dc.contributor.author Shirshikova T.
dc.contributor.author Skipina I.
dc.contributor.author Bogomolnaya L.
dc.date.accessioned 2018-09-19T23:12:17Z
dc.date.available 2018-09-19T23:12:17Z
dc.date.issued 2016
dc.identifier.issn 2191-1630
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/146059
dc.description.abstract © 2016, Springer Science+Business Media New York.Gram-negative bacterium Serratia marcescens is a well-known environmental microorganism and the accepted clinical pathogen causing nosocomial infections. It attracts more attention in recent years due to the emergence of strains with multiple drug resistance. Standard recombinant techniques are difficult to apply to S. marcescens due to the presence of numerous hydrolytic enzymes, in particular, extracellular nuclease and restriction endonuclease, which degrade transforming DNAs. We overcame this obstacle by utilizing restrictionless nuclease-deficient mutant strain S. marcescens TT392. As a proof of principal, in this genetic background, we generated a knockout strain with deletion of macAB locus using lambda red technology. The resulting mutation could be easily moved to a new genetic background by generalized phage transduction. This strategy provides a good tool for evaluation of S. marcescens pathogenic potential.
dc.relation.ispartofseries BioNanoScience
dc.subject Efflux pump
dc.subject Lambda red recombinase
dc.subject MacAB
dc.subject PCR
dc.subject Serratia marcescens
dc.title Inactivation of Chromosomal Genes in Serratia marcescens
dc.type Article
dc.relation.ispartofseries-issue 4
dc.relation.ispartofseries-volume 6
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 376
dc.source.id SCOPUS21911630-2016-6-4-SID84999861292

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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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