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Effect of Adenoviral Transduction of Hepatic Stellate Cells with Adv5-optHGF-RFP on their Phenotype

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dc.contributor.author Shafigullina A.
dc.contributor.author Zaikina E.
dc.contributor.author Garanina E.
dc.contributor.author Sakhapov D.
dc.contributor.author Titova A.
dc.contributor.author Mavlikeev M.
dc.contributor.author Rizvanov A.
dc.contributor.author Gumerova A.
dc.contributor.author Kiassov A.
dc.date.accessioned 2018-09-19T22:59:08Z
dc.date.available 2018-09-19T22:59:08Z
dc.date.issued 2017
dc.identifier.issn 2191-1630
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/145868
dc.description.abstract © 2016, Springer Science+Business Media New York.Adenovirus is a promising gene delivery vector that has a high efficiency and relative simplicity of construction. These advantages make this system attractive for diverse research applications. In this project we transduced hepatics stellatecells (HSC) with adenoviral vector containing genes of hepatocytes growth factor (HGF) and red fluorescent protein (RFP) as a reporter (Adv5-optHGF-RFP), which let us to visualize the transduced cells. Further changes of phenotype were studied by real-time PCR and immunocytochemical staining. According to our results, most of the cells were transduced and demonstrated stable expression of RFP. Effectiveness of the transduction was also confirmed by a high expression of HGF (1200 times higher than in control HSC culture). Adenoviral transduction of HSC with HGF gene did not change their morphology, but stimulated expression of HSC marker desmin, leading to HSC activation (α-smooth muscle actin) and appearance of α-fetoprotein, one of the hepatoblasts markers. During the entire experiment, there were Ki-67+ cells, meaning that proliferation of transduced HSC was not affected. Thus, adenoviral transduction of HSC with Adv5-optHGF-RFP is a good gene delivery system with a stimulating effect on HSC.
dc.relation.ispartofseries BioNanoScience
dc.subject Adenovirus
dc.subject Differentiation
dc.subject Hepatic stellate cells
dc.subject Transduction
dc.title Effect of Adenoviral Transduction of Hepatic Stellate Cells with Adv5-optHGF-RFP on their Phenotype
dc.type Article
dc.relation.ispartofseries-issue 2
dc.relation.ispartofseries-volume 7
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 419
dc.source.id SCOPUS21911630-2017-7-2-SID85019065278


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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