Kazan Federal University Digital Repository

Isolation and purification of staphylococcus aureus hibernationpromoting factor inactivating of the ribosome

Show simple item record

dc.contributor.author Ayupov R.
dc.contributor.author Khusainov I.
dc.contributor.author Validov S.
dc.contributor.author Yusupova G.
dc.contributor.author Yusupov M.
dc.date.accessioned 2018-09-19T22:51:41Z
dc.date.available 2018-09-19T22:51:41Z
dc.date.issued 2016
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/145647
dc.description.abstract © 2016, International Journal of Pharmacy and Technology. All rights reserved.In Staphylococcus aureus hibernation-promoting factor (SaHPF) binds to ribosomes depleting translation and turning metabolism of this pathogenic bacteria in energy saving mode. This phenomenon can be recognized as unspecific resistance mechanism, which is exploited by staphylococci to persist in presence of large spectrum of antibiotics. Investigation of structure of SaHPF and its interaction mechanisms with ribosome can aid to design new drugs, rendering S. aureus sensitive to antibiotics. We developed techniques for SaHPF purification, which provide this protein in appropriate quantity and quality for further structural analysis. Gene Sahpf has been cloned in expression vector pGS21а. To facilitate purification process His-tag coding sequence was introduced into Sahpf gene to generate SaHPF protein with six histidine residues on C-terminus. Eschercha coli strain BL21star (DE3) harboring Sahpf::pGS21а was used for the protein expression. The protein purification was conducted using affinity chromatography with Ni-NTA resin followed by size-exclusion chromatography in Superdex-75. High purification rate and homogeneity of SaHPF preparations obtained allows further study of this protein using nuclear magnetic resonance and development of crystallization procedure for X-ray analysis.
dc.subject Hibernation-promoting factor Staphylococcus aureus
dc.subject Isolation and purification protein
dc.subject NMR
dc.subject Ribosome
dc.subject Structure
dc.title Isolation and purification of staphylococcus aureus hibernationpromoting factor inactivating of the ribosome
dc.type Article
dc.relation.ispartofseries-issue 2
dc.relation.ispartofseries-volume 8
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 14392
dc.source.id SCOPUS-2016-8-2-SID84978512552


Files in this item

This item appears in the following Collection(s)

  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

Show simple item record

Search DSpace


Advanced Search

Browse

My Account

Statistics