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Acetylcholine-induced inhibition of presynaptic calcium signals and transmitter release in the frog neuromuscular junction

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dc.contributor.author Khaziev E.
dc.contributor.author Samigullin D.
dc.contributor.author Zhilyakov N.
dc.contributor.author Fatikhov N.
dc.contributor.author Bukharaeva E.
dc.contributor.author Verkhratsky A.
dc.contributor.author Nikolsky E.
dc.date.accessioned 2018-09-19T22:51:20Z
dc.date.available 2018-09-19T22:51:20Z
dc.date.issued 2016
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/145635
dc.description.abstract © 2016 Khaziev, Samigullin, Zhilyakov, Fatikhov, Bukharaeva, Verkhratsky and Nikolsky.Acetylcholine (ACh), released from axonal terminals of motor neurons in neuromuscular junctions regulates the efficacy of neurotransmission through activation of presynaptic nicotinic and muscarinic autoreceptors. Receptor-mediated presynaptic regulation could reflect either direct action on exocytotic machinery or modulation of Ca2+ entry and resulting intra-terminal Ca2+ dynamics. We have measured free intra-terminal cytosolic Ca2+ ([Ca2+]i) using Oregon-Green 488 microfluorimetry, in parallel with voltage-clamp recordings of spontaneous (mEPC) and evoked (EPC) postsynaptic currents in post-junctional skeletal muscle fiber. Activation of presynaptic muscarinic and nicotinic receptors with exogenous acetylcholine and its non-hydrolized analog carbachol reduced amplitude of the intra-terminal [Ca2+]i transients and decreased quantal content (calculated by dividing the area under EPC curve by the area under mEPC curve). Pharmacological analysis revealed the role of muscarinic receptors of M2 subtype as well as d-tubocurarine-sensitive nicotinic receptor in presynaptic modulation of [Ca2+]i transients. Modulation of synaptic transmission efficacy by ACh receptors was completely eliminated by pharmacological inhibition of N-type Ca2+ channels. We conclude that ACh receptor-mediated reduction of Ca2+ entry into the nerve terminal through N-type Ca2+ channels represents one of possible mechanism of presynaptic modulation in frog neuromuscular junction.
dc.subject Calcium transient
dc.subject Muscarinic receptors
dc.subject N-type Ca channels
dc.subject Neuromuscular synapse
dc.subject Nicotinic receptors
dc.subject Presynaptic acetylcholine receptors
dc.subject Quantum secretion of acetylcholine
dc.title Acetylcholine-induced inhibition of presynaptic calcium signals and transmitter release in the frog neuromuscular junction
dc.type Article
dc.relation.ispartofseries-issue DEC
dc.relation.ispartofseries-volume 7
dc.collection Публикации сотрудников КФУ
dc.source.id SCOPUS-2016-7--SID85009352866


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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