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dc.contributor.author | Rozov A. | |
dc.contributor.author | Demeshkina N. | |
dc.contributor.author | Khusainov I. | |
dc.contributor.author | Westhof E. | |
dc.contributor.author | Yusupov M. | |
dc.contributor.author | Yusupova G. | |
dc.date.accessioned | 2018-09-19T22:51:07Z | |
dc.date.available | 2018-09-19T22:51:07Z | |
dc.date.issued | 2016 | |
dc.identifier.uri | https://dspace.kpfu.ru/xmlui/handle/net/145628 | |
dc.description.abstract | Posttranscriptional modifications at the wobble position of transfer RNAs play a substantial role in deciphering the degenerate genetic code on the ribosome. The number and variety of modifications suggest different mechanisms of action during messenger RNA decoding, of which only a few were described so far. Here, on the basis of several 70S ribosome complex X-ray structures, we demonstrate how Escherichia coli tRNA Lys UUU with hypermodified 5-methylaminomethyl-2-thiouridine (mnm 5 s 2 U) at the wobble position discriminates between cognate codons AAA and AAG, and near-cognate stop codon UAA or isoleucine codon AUA, with which it forms pyrimidine-pyrimidine mismatches. We show that mnm 5 s 2 U forms an unusual pair with guanosine at the wobble position that expands general knowledge on the degeneracy of the genetic code and specifies a powerful role of tRNA modifications in translation. Our models consolidate the translational fidelity mechanism proposed previously where the steric complementarity and shape acceptance dominate the decoding mechanism. | |
dc.title | Novel base-pairing interactions at the tRNA wobble position crucial for accurate reading of the genetic code | |
dc.type | Article | |
dc.relation.ispartofseries-volume | 7 | |
dc.collection | Публикации сотрудников КФУ | |
dc.source.id | SCOPUS-2016-7-SID84955515428 |