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dc.contributor.author | Lebedeva A. | |
dc.contributor.author | Zakharchenko N. | |
dc.contributor.author | Trubnikova E. | |
dc.contributor.author | Medvedeva O. | |
dc.contributor.author | Kuznetsova T. | |
dc.contributor.author | Masgutova G. | |
dc.contributor.author | Zylkova M. | |
dc.contributor.author | Buryanov Y. | |
dc.contributor.author | Belous A. | |
dc.date.accessioned | 2018-09-19T22:30:44Z | |
dc.date.available | 2018-09-19T22:30:44Z | |
dc.date.issued | 2017 | |
dc.identifier.uri | https://dspace.kpfu.ru/xmlui/handle/net/145296 | |
dc.description.abstract | Procedure of manufacturing K. pinnata water extracts containing cecropin P1 (CecP1) from the formerly described transgenic plants is established. It included incubation of leaves at +4°C for 7 days, mechanical homogenization of leaves using water as extraction solvent, and heating at +70°C for inactivating plant enzymes. Yield of CecP1 (after heating and sterilizing filtration) was 0.3% of total protein in the extract. The water extract of K. pinnata + CecP1 exhibits favorable effect on healing of wounds infected with S. aureus (equal to Cefazolin) and with a combination of S. aureus with P. aeruginosa (better than Cefazolin). Wild-type K. pinnata extract exhibited evident microbicide activity against S. aureus with P. aeruginosa but it was substantially strengthened in K. pinnata + CecP1 extract. K. pinnata extracts (both wild-type and transgenic) did not exhibit general toxicity and accelerated wound recovery. Due to immunomodulating activity, wild-type K. pinnata extract accelerated granulation of the wound bed and marginal epithelialization even better than K. pinnata + CecP1 extract. Immunomodulating and microbicide activity of K. pinnata synergizes with microbicide activity of CecP1 accelerating elimination of bacteria. | |
dc.title | Bactericide, Immunomodulating, and Wound Healing Properties of Transgenic Kalanchoe pinnata Synergize with Antimicrobial Peptide Cecropin P1 In Vivo | |
dc.type | Article | |
dc.relation.ispartofseries-volume | 2017 | |
dc.collection | Публикации сотрудников КФУ | |
dc.relation.startpage | 4645701 | |
dc.source.id | SCOPUS-2017-2017-SID85013149855 |