dc.contributor.author |
Lebedeva A. |
|
dc.contributor.author |
Zakharchenko N. |
|
dc.contributor.author |
Trubnikova E. |
|
dc.contributor.author |
Medvedeva O. |
|
dc.contributor.author |
Kuznetsova T. |
|
dc.contributor.author |
Masgutova G. |
|
dc.contributor.author |
Zylkova M. |
|
dc.contributor.author |
Buryanov Y. |
|
dc.contributor.author |
Belous A. |
|
dc.date.accessioned |
2018-09-19T22:30:44Z |
|
dc.date.available |
2018-09-19T22:30:44Z |
|
dc.date.issued |
2017 |
|
dc.identifier.uri |
https://dspace.kpfu.ru/xmlui/handle/net/145296 |
|
dc.description.abstract |
Procedure of manufacturing K. pinnata water extracts containing cecropin P1 (CecP1) from the formerly described transgenic plants is established. It included incubation of leaves at +4°C for 7 days, mechanical homogenization of leaves using water as extraction solvent, and heating at +70°C for inactivating plant enzymes. Yield of CecP1 (after heating and sterilizing filtration) was 0.3% of total protein in the extract. The water extract of K. pinnata + CecP1 exhibits favorable effect on healing of wounds infected with S. aureus (equal to Cefazolin) and with a combination of S. aureus with P. aeruginosa (better than Cefazolin). Wild-type K. pinnata extract exhibited evident microbicide activity against S. aureus with P. aeruginosa but it was substantially strengthened in K. pinnata + CecP1 extract. K. pinnata extracts (both wild-type and transgenic) did not exhibit general toxicity and accelerated wound recovery. Due to immunomodulating activity, wild-type K. pinnata extract accelerated granulation of the wound bed and marginal epithelialization even better than K. pinnata + CecP1 extract. Immunomodulating and microbicide activity of K. pinnata synergizes with microbicide activity of CecP1 accelerating elimination of bacteria. |
|
dc.title |
Bactericide, Immunomodulating, and Wound Healing Properties of Transgenic Kalanchoe pinnata Synergize with Antimicrobial Peptide Cecropin P1 In Vivo |
|
dc.type |
Article |
|
dc.relation.ispartofseries-volume |
2017 |
|
dc.collection |
Публикации сотрудников КФУ |
|
dc.relation.startpage |
4645701 |
|
dc.source.id |
SCOPUS-2017-2017-SID85013149855 |
|