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Development of a recombinant immunotoxin for the immunotherapy of autoreactive lymphocytes expressing MOG-specific BCRs

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dc.contributor.author Stepanov A.
dc.contributor.author Belyy A.
dc.contributor.author Kasheverov I.
dc.contributor.author Rybinets A.
dc.contributor.author Dronina M.
dc.contributor.author Dyachenko I.
dc.contributor.author Murashev A.
dc.contributor.author Knorre V.
dc.contributor.author Sakharov D.
dc.contributor.author Ponomarenko N.
dc.contributor.author Tsetlin V.
dc.contributor.author Tonevitsky A.
dc.contributor.author Deyev S.
dc.contributor.author Belogurov A.
dc.contributor.author Gabibov A.
dc.date.accessioned 2018-09-19T21:53:25Z
dc.date.available 2018-09-19T21:53:25Z
dc.date.issued 2016
dc.identifier.issn 0141-5492
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/144454
dc.description.abstract © 2016, Springer Science+Business Media Dordrecht.Objective: Myelin oligodendrocyte glycoprotein (MOG) is one of the major autoantigens in multiple sclerosis (MS), therefore selective depletion of autoreactive lymphocytes exposing MOG-specific B cell receptors (BCRs) would be beneficial in terms of MS treatment. Results: Using E. coli we generated an efficient protocol for the purification of the recombinant immunotoxin DT-MOG composed of the extracellular Ig-like domain of MOG fused in frame with the catalytic and translocation subunits of diphtheria toxin (DT, Corynebacterium diphtheriae) under native conditions with a final yield of 1.5 mg per liter of culture medium. Recombinant DT-MOG was recognized in vitro by MOG-reactive antibodies and has catalytic activity comparable with wild-type DT. Conclusion: Enhanced pharmacokinetics (mean residence time in the bloodstream of 61 min) and minimized diminished nonspecific toxicity (LD50 = 1.76 mg/kg) of the DT-MOG makes it a potential candidate for the immunotherapy of MS.
dc.relation.ispartofseries Biotechnology Letters
dc.subject Diphtheria toxin
dc.subject Immunotoxin
dc.subject Myelin oligodendrocyte glycoprotein
dc.subject Prokaryotic expression
dc.title Development of a recombinant immunotoxin for the immunotherapy of autoreactive lymphocytes expressing MOG-specific BCRs
dc.type Article
dc.relation.ispartofseries-issue 7
dc.relation.ispartofseries-volume 38
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 1173
dc.source.id SCOPUS01415492-2016-38-7-SID84964343985


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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