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Causal interrogation of neuronal networks and behavior through virally transduced ivermectin receptors

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dc.contributor.author Obenhaus H.
dc.contributor.author Rozov A.
dc.contributor.author Bertocchi I.
dc.contributor.author Tang W.
dc.contributor.author Kirsch J.
dc.contributor.author Betz H.
dc.contributor.author Sprengel R.
dc.date.accessioned 2018-09-19T21:31:40Z
dc.date.available 2018-09-19T21:31:40Z
dc.date.issued 2016
dc.identifier.issn 1662-5099
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/144092
dc.description.abstract © 2016 Obenhaus, Rozov, Bertocchi, Tang, Kirsch, Betz and Sprengel.The causal interrogation of neuronal networks involved in specific behaviors requires the spatially and temporally controlled modulation of neuronal activity. For long-term manipulation of neuronal activity, chemogenetic tools provide a reasonable alternative to short-term optogenetic approaches. Here we show that virus mediated gene transfer of the ivermectin (IVM) activated glycine receptor mutant GlyRα1AG can be used for the selective and reversible silencing of specific neuronal networks in mice. In the striatum, dorsal hippocampus, and olfactory bulb, GlyRα1AG promoted IVM dependent effects in representative behavioral assays. Moreover, GlyRα1AG mediated silencing had a strong and reversible impact on neuronal ensemble activity and c-Fos activation in the olfactory bulb. Together our results demonstrate that long-term, reversible and re- inducible neuronal silencing via GlyRα1AG is a promising tool for the interrogation of network mechanisms underlying the control of behavior and memory formation.
dc.relation.ispartofseries Frontiers in Molecular Neuroscience
dc.subject Glycine receptor
dc.subject Ivermectin
dc.subject Neuronal silencing
dc.subject Odor discrimination
dc.subject RAAV
dc.title Causal interrogation of neuronal networks and behavior through virally transduced ivermectin receptors
dc.type Article
dc.relation.ispartofseries-issue AUG
dc.relation.ispartofseries-volume 9
dc.collection Публикации сотрудников КФУ
dc.source.id SCOPUS16625099-2016-9--SID84991574358


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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