dc.contributor.author |
Sogame Y. |
|
dc.contributor.author |
Okada J. |
|
dc.contributor.author |
Kikuta S. |
|
dc.contributor.author |
Miyata Y. |
|
dc.contributor.author |
Cornette R. |
|
dc.contributor.author |
Gusev O. |
|
dc.contributor.author |
Kikawada T. |
|
dc.date.accessioned |
2018-09-19T21:16:03Z |
|
dc.date.available |
2018-09-19T21:16:03Z |
|
dc.date.issued |
2017 |
|
dc.identifier.issn |
1431-0651 |
|
dc.identifier.uri |
https://dspace.kpfu.ru/xmlui/handle/net/143698 |
|
dc.description.abstract |
© 2016, Springer Japan.Larvae of the African midge Polypedilum vanderplanki show extreme desiccation tolerance, known as anhydrobiosis. Recently, the cultured cell line Pv11 was derived from this species; Pv11 cells can be preserved in the dry state for over 6 months and retain their proliferation potential. Here, we attempted to expand the use of Pv11 cells as a model to investigate the mechanisms underlying anhydrobiosis in P. vanderplanki. A newly developed vector comprising a constitutive promoter for the PvGapdh gene allowed the expression of exogenous proteins in Pv11 cells. Using this vector, a stable Pv11 cell line expressing green fluorescence protein (GFP) was established and retained desiccation tolerance. Gene silencing with GFP-specific siRNAs significantly suppressed GFP expression to approximately 7.5–34.6% of that in the non-siRNA-transfected GFP stable line. Establishment of these functional assays will enable Pv11 cells to be utilized as an effective tool to investigate the molecular mechanisms underlying anhydrobiosis. |
|
dc.relation.ispartofseries |
Extremophiles |
|
dc.subject |
Anhydrobiosis |
|
dc.subject |
Desiccation tolerant cells |
|
dc.subject |
Gene expression system |
|
dc.subject |
RNAi |
|
dc.title |
Establishment of gene transfer and gene silencing methods in a desiccation-tolerant cell line, Pv11 |
|
dc.type |
Article |
|
dc.relation.ispartofseries-issue |
1 |
|
dc.relation.ispartofseries-volume |
21 |
|
dc.collection |
Публикации сотрудников КФУ |
|
dc.relation.startpage |
65 |
|
dc.source.id |
SCOPUS14310651-2017-21-1-SID84991660129 |
|