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dc.contributor.author | Sogame Y. | |
dc.contributor.author | Okada J. | |
dc.contributor.author | Kikuta S. | |
dc.contributor.author | Miyata Y. | |
dc.contributor.author | Cornette R. | |
dc.contributor.author | Gusev O. | |
dc.contributor.author | Kikawada T. | |
dc.date.accessioned | 2018-09-19T21:16:03Z | |
dc.date.available | 2018-09-19T21:16:03Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 1431-0651 | |
dc.identifier.uri | https://dspace.kpfu.ru/xmlui/handle/net/143698 | |
dc.description.abstract | © 2016, Springer Japan.Larvae of the African midge Polypedilum vanderplanki show extreme desiccation tolerance, known as anhydrobiosis. Recently, the cultured cell line Pv11 was derived from this species; Pv11 cells can be preserved in the dry state for over 6 months and retain their proliferation potential. Here, we attempted to expand the use of Pv11 cells as a model to investigate the mechanisms underlying anhydrobiosis in P. vanderplanki. A newly developed vector comprising a constitutive promoter for the PvGapdh gene allowed the expression of exogenous proteins in Pv11 cells. Using this vector, a stable Pv11 cell line expressing green fluorescence protein (GFP) was established and retained desiccation tolerance. Gene silencing with GFP-specific siRNAs significantly suppressed GFP expression to approximately 7.5–34.6% of that in the non-siRNA-transfected GFP stable line. Establishment of these functional assays will enable Pv11 cells to be utilized as an effective tool to investigate the molecular mechanisms underlying anhydrobiosis. | |
dc.relation.ispartofseries | Extremophiles | |
dc.subject | Anhydrobiosis | |
dc.subject | Desiccation tolerant cells | |
dc.subject | Gene expression system | |
dc.subject | RNAi | |
dc.title | Establishment of gene transfer and gene silencing methods in a desiccation-tolerant cell line, Pv11 | |
dc.type | Article | |
dc.relation.ispartofseries-issue | 1 | |
dc.relation.ispartofseries-volume | 21 | |
dc.collection | Публикации сотрудников КФУ | |
dc.relation.startpage | 65 | |
dc.source.id | SCOPUS14310651-2017-21-1-SID84991660129 |