dc.contributor.author |
Kostianets O. |
|
dc.contributor.author |
Shyyan M. |
|
dc.contributor.author |
Antoniuk S. |
|
dc.contributor.author |
Filonenko V. |
|
dc.contributor.author |
Kiyamova R. |
|
dc.date.accessioned |
2018-09-19T21:14:33Z |
|
dc.date.available |
2018-09-19T21:14:33Z |
|
dc.date.issued |
2017 |
|
dc.identifier.issn |
1354-750X |
|
dc.identifier.uri |
https://dspace.kpfu.ru/xmlui/handle/net/143670 |
|
dc.description.abstract |
© 2016 Informa UK Limited, trading as Taylor & Francis Group.Content: Identification of panel of SEREX-defined antigens for breast cancer autoantibodies profile detection. Objective: To create panel of antigens that can differentiate breast cancer patients and healthy individuals. Methods: SEREX (serological analysis of cDNA expression libraries) method, ELISA (enzyme-linked immunosorbent assay), qPCR (quantitative polymerase chain reaction). Results: In large-scale screening of 16 SEREX-antigens by sera of breast cancer patients and healthy donors, a combination of six antigens (RAD50, PARD3, SPP1, SAP30BP, NY-BR-62 and NY-CO-58) was identified, which can differentiate breast cancer patients and healthy donors with 70% sensitivity and 91% specificity. Elevated mRNA expression of SPP1 gene was revealed in breast tumors (2–7-fold) that correlated with SPP1 antigen immunoreactivity in autologous patients’ sera. Conclusions: The new panel of six SEREX-antigens was proposed, which enables creation of serological assay for breast cancer diagnostics and/or prognosis. |
|
dc.relation.ispartofseries |
Biomarkers |
|
dc.subject |
antigenic panel |
|
dc.subject |
autoantibody |
|
dc.subject |
breast cancer |
|
dc.subject |
Tumor-associated antigens |
|
dc.title |
Panel of SEREX-defined antigens for breast cancer autoantibodies profile detection |
|
dc.type |
Article |
|
dc.relation.ispartofseries-issue |
2 |
|
dc.relation.ispartofseries-volume |
22 |
|
dc.collection |
Публикации сотрудников КФУ |
|
dc.relation.startpage |
149 |
|
dc.source.id |
SCOPUS1354750X-2017-22-2-SID84994890034 |
|