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Purification of recombinant extracellular proteases from Bacillus pumilus for ß-amyloid peptide cleavage
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| dc.contributor.author | Toymentseva A. | |
| dc.contributor.author | Danilova I. | |
| dc.contributor.author | Tihonova A. | |
| dc.contributor.author | Sharipova M. | |
| dc.contributor.author | Balaban N. | |
| dc.date.accessioned | 2018-09-19T20:57:53Z | |
| dc.date.available | 2018-09-19T20:57:53Z | |
| dc.date.issued | 2016 | |
| dc.identifier.issn | 1068-1620 | |
| dc.identifier.uri | https://dspace.kpfu.ru/xmlui/handle/net/143465 | |
| dc.description.abstract | © 2016 Pleiades Publishing, Ltd. Using the expression vector pGP382 containing a constitutive promoter (P degQ36 ) and an affinity tag (Strep-tag), we have obtained highly purified recombinant Bacillus pumilus 3-19 proteinases with different substrate specificities: glutamylendopeptidase (GseBp), subtilisin-like protease (AprBp), and metalloendopeptidase (MprBp). The products of the hydrolysis of the ß-amyloid peptide by the bacterial proteases from B. pumilus have been studied. The findings on the potential of the practical application of these bacterial enzymes as the agents preventing the development of the Alzheimer's disease are presented. | |
| dc.relation.ispartofseries | Russian Journal of Bioorganic Chemistry | |
| dc.subject | affinity chromatography | |
| dc.subject | Bacillus pumilus 3-19 | |
| dc.subject | biological activity | |
| dc.subject | expression vector | |
| dc.subject | glutamylendopeptidase | |
| dc.subject | metalloendopeptidase | |
| dc.subject | subtilisin-like protease | |
| dc.title | Purification of recombinant extracellular proteases from Bacillus pumilus for ß-amyloid peptide cleavage | |
| dc.type | Article | |
| dc.relation.ispartofseries-issue | 1 | |
| dc.relation.ispartofseries-volume | 42 | |
| dc.collection | Публикации сотрудников КФУ | |
| dc.relation.startpage | 53 | |
| dc.source.id | SCOPUS10681620-2016-42-1-SID84957573145 |
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Публикации сотрудников КФУ Scopus [24551]
Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.