dc.contributor.author |
Zhmurov A. |
|
dc.contributor.author |
Protopopova A. |
|
dc.contributor.author |
Litvinov R. |
|
dc.contributor.author |
Zhukov P. |
|
dc.contributor.author |
Mukhitov A. |
|
dc.contributor.author |
Weisel J. |
|
dc.contributor.author |
Barsegov V. |
|
dc.date.accessioned |
2018-09-19T20:44:24Z |
|
dc.date.available |
2018-09-19T20:44:24Z |
|
dc.date.issued |
2016 |
|
dc.identifier.issn |
0969-2126 |
|
dc.identifier.uri |
https://dspace.kpfu.ru/xmlui/handle/net/143200 |
|
dc.description.abstract |
© 2016 Elsevier LtdFibrin is a filamentous network made in blood to stem bleeding; it forms when fibrinogen is converted into fibrin monomers that self-associate into oligomers and then to polymers. To gather structural insights into fibrin formation and properties, we combined high-resolution atomic force microscopy of fibrin(ogen) oligomers and molecular modeling of crystal structures of fibrin(ogen) and its fragments. We provided a structural basis for the intermolecular flexibility of single-stranded fibrin(ogen) oligomers and identified a hinge region at the D:D inter-monomer junction. Following computational reconstruction of the missing portions, we recreated the full-atomic structure of double-stranded fibrin oligomers that was validated by quantitative comparison with the experimental images. We characterized previously unknown intermolecular binding contacts at the D:D and D:E:D interfaces, which drive oligomerization and reinforce the intra- and inter-strand connections in fibrin besides the known knob-hole bonds. The atomic models provide valuable insights into the submolecular mechanisms of fibrin polymerization. |
|
dc.relation.ispartofseries |
Structure |
|
dc.subject |
D:D interface |
|
dc.subject |
D:E:D interface |
|
dc.subject |
fibrin |
|
dc.subject |
fibrin oligomers |
|
dc.subject |
fibrin polymerization |
|
dc.subject |
fibrinogen |
|
dc.subject |
MD simulations on a GPU |
|
dc.title |
Structural Basis of Interfacial Flexibility in Fibrin Oligomers |
|
dc.type |
Article |
|
dc.relation.ispartofseries-issue |
11 |
|
dc.relation.ispartofseries-volume |
24 |
|
dc.collection |
Публикации сотрудников КФУ |
|
dc.relation.startpage |
1907 |
|
dc.source.id |
SCOPUS09692126-2016-24-11-SID84994378076 |
|