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dc.contributor.author | Zhmurov A. | |
dc.contributor.author | Protopopova A. | |
dc.contributor.author | Litvinov R. | |
dc.contributor.author | Zhukov P. | |
dc.contributor.author | Mukhitov A. | |
dc.contributor.author | Weisel J. | |
dc.contributor.author | Barsegov V. | |
dc.date.accessioned | 2018-09-19T20:44:24Z | |
dc.date.available | 2018-09-19T20:44:24Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 0969-2126 | |
dc.identifier.uri | https://dspace.kpfu.ru/xmlui/handle/net/143200 | |
dc.description.abstract | © 2016 Elsevier LtdFibrin is a filamentous network made in blood to stem bleeding; it forms when fibrinogen is converted into fibrin monomers that self-associate into oligomers and then to polymers. To gather structural insights into fibrin formation and properties, we combined high-resolution atomic force microscopy of fibrin(ogen) oligomers and molecular modeling of crystal structures of fibrin(ogen) and its fragments. We provided a structural basis for the intermolecular flexibility of single-stranded fibrin(ogen) oligomers and identified a hinge region at the D:D inter-monomer junction. Following computational reconstruction of the missing portions, we recreated the full-atomic structure of double-stranded fibrin oligomers that was validated by quantitative comparison with the experimental images. We characterized previously unknown intermolecular binding contacts at the D:D and D:E:D interfaces, which drive oligomerization and reinforce the intra- and inter-strand connections in fibrin besides the known knob-hole bonds. The atomic models provide valuable insights into the submolecular mechanisms of fibrin polymerization. | |
dc.relation.ispartofseries | Structure | |
dc.subject | D:D interface | |
dc.subject | D:E:D interface | |
dc.subject | fibrin | |
dc.subject | fibrin oligomers | |
dc.subject | fibrin polymerization | |
dc.subject | fibrinogen | |
dc.subject | MD simulations on a GPU | |
dc.title | Structural Basis of Interfacial Flexibility in Fibrin Oligomers | |
dc.type | Article | |
dc.relation.ispartofseries-issue | 11 | |
dc.relation.ispartofseries-volume | 24 | |
dc.collection | Публикации сотрудников КФУ | |
dc.relation.startpage | 1907 | |
dc.source.id | SCOPUS09692126-2016-24-11-SID84994378076 |