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Muscarinic cholinoreceptors (M1-, M2-, M3- and M4-type) modulate the acetylcholine secretion in the frog neuromuscular junction

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dc.contributor.author Tsentsevitsky A.
dc.contributor.author Kovyazina I.
dc.contributor.author Nurullin L.
dc.contributor.author Nikolsky E.
dc.date.accessioned 2018-09-19T20:30:48Z
dc.date.available 2018-09-19T20:30:48Z
dc.date.issued 2017
dc.identifier.issn 0304-3940
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/142968
dc.description.abstract © 2017 Elsevier B.V.Muscarinic cholinoreceptors regulate the neurosecretion process in vertebrate neuromuscular junctions. The diversity of muscarinic effects on acetylcholine (ACh) secretion may be attributed to the different muscarinic subtypes involved in this process. In the present study, the location of five muscarinic receptor subtypes (M1, M2, M3, M4 and M5) on the motor nerve terminals of frog cutaneous pectoris muscle was shown using specific polyclonal antibodies. The modulatory roles of these receptors were investigated via assessment of the effects of muscarine and specific muscarinic antagonists on the quantal content of endplate currents (EPCs) and the time course of secretion, which was estimated from the distribution of “real” synaptic delays of EPCs recorded in a low Ca2+/high Mg2+ solution. The agonist muscarine decreased the EPC quantal content and synchronized the release process. The depressing action of muscarine on the EPC quantal content was abolished only by pretreatment of the preparation with the M3 blockers 4-DAMP (1,1-Dimethyl-4-diphenylacetoxypiperidinium iodide) and J 104129 fumarate ((αR)-α-Cyclopentyl-α-hydroxy-N-[1-(4-methyl-3-pentenyl)-4-piperidinyl]benzeneacetamide fumarate). Moreover, antagonists of the M1, M2, M3 and M4 receptors per se diminished the intensity of secretion, which suggests a putative up-regulation of the release by endogenous ACh.
dc.relation.ispartofseries Neuroscience Letters
dc.subject Acetylcholine
dc.subject Cholinoreceptor
dc.subject Endplate current
dc.subject Muscarine
dc.subject Neuromuscular junction
dc.title Muscarinic cholinoreceptors (M1-, M2-, M3- and M4-type) modulate the acetylcholine secretion in the frog neuromuscular junction
dc.type Article
dc.relation.ispartofseries-volume 649
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 62
dc.source.id SCOPUS03043940-2017-649-SID85017552111


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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