Kazan Federal University Digital Repository

Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity

Show simple item record

dc.contributor.author Terekhov S.
dc.contributor.author Smirnov I.
dc.contributor.author Stepanova A.
dc.contributor.author Bobik T.
dc.contributor.author Mokrushina Y.
dc.contributor.author Ponomarenko N.
dc.contributor.author Belogurov A.
dc.contributor.author Rubtsova M.
dc.contributor.author Kartseva O.
dc.contributor.author Gomzikova M.
dc.contributor.author Moskovtsev A.
dc.contributor.author Bukatin A.
dc.contributor.author Dubina M.
dc.contributor.author Kostryukova E.
dc.contributor.author Babenko V.
dc.contributor.author Vakhitova M.
dc.contributor.author Manolov A.
dc.contributor.author Malakhova M.
dc.contributor.author Kornienko M.
dc.contributor.author Tyakht A.
dc.contributor.author Vanyushkina A.
dc.contributor.author Ilina E.
dc.contributor.author Masson P.
dc.contributor.author Gabibov A.
dc.contributor.author Altman S.
dc.date.accessioned 2018-09-19T20:23:01Z
dc.date.available 2018-09-19T20:23:01Z
dc.date.issued 2017
dc.identifier.issn 0027-8424
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/142853
dc.description.abstract © 2017, National Academy of Sciences. All rights reserved.Ultrahigh-throughput screening (uHTS) techniques can identify unique functionality from millions of variants. To mimic the natural selection mechanisms that occur by compartmentalization in vivo, we developed a technique based on single-cell encapsulation in droplets of a monodisperse microfluidic double water-in-oil-in-water emulsion (MDE). Biocompatible MDE enables in-droplet cultivation of different living species. The combination of droplet-generating machinery with FACS followed by next-generation sequencing and liquid chromatography-mass spectrometry analysis of the secretomes of encapsulated organisms yielded detailed genotype/phenotype descriptions. This platform was probed with uHTS for biocatalysts anchored to yeast with enrichment close to the theoretically calculated limit and cell-to-cell interactions. MDE-FACS allowed the identification of human butyrylcholinesterase mutants that undergo self-reactivation after inhibition by the organophosphorus agent paraoxon. The versatility of the platform allowed the identification of bacteria, including slow-growing oral microbiota species that suppress the growth of a common pathogen, Staphylococcus aureus, and predicted which genera were associated with inhibitory activity.
dc.relation.ispartofseries Proceedings of the National Academy of Sciences of the United States of America
dc.subject Butyrylcholinesterase
dc.subject Cell-cell interactions
dc.subject Microfluidic encapsulation
dc.subject Staphylococcus aureus
dc.subject Ultrahigh-throughput screening
dc.title Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity
dc.type Article
dc.relation.ispartofseries-issue 10
dc.relation.ispartofseries-volume 114
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 2550
dc.source.id SCOPUS00278424-2017-114-10-SID85014656468


Files in this item

This item appears in the following Collection(s)

  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

Show simple item record

Search DSpace


Advanced Search

Browse

My Account

Statistics