dc.contributor.author |
Dyukova E. |
|
dc.contributor.author |
Schreckenberg R. |
|
dc.contributor.author |
Arens C. |
|
dc.contributor.author |
Sitdikova G. |
|
dc.contributor.author |
Schlüter K. |
|
dc.date.accessioned |
2018-09-19T20:14:12Z |
|
dc.date.available |
2018-09-19T20:14:12Z |
|
dc.date.issued |
2017 |
|
dc.identifier.issn |
0021-9541 |
|
dc.identifier.uri |
https://dspace.kpfu.ru/xmlui/handle/net/142721 |
|
dc.description.abstract |
© 2016 Wiley Periodicals, Inc.Nitric oxide (NO)-deficiency as it occurs during endothelial dysfunction activates the endothelin-1 (ET-1) system and increases the expression of receptor activity modifying protein (RAMP)-1 that acts as a chaperon for calcium-sensing receptors (CaR) that have recently been identified to improve cardiac function. Here, we hypothesized that ET-1 increases the cardiac expression of CaR and thereby induces an adaptive type of hypertrophy. Expressions of RAMP-1, endothelin receptors, and CaR were analyzed by RT-PCR in left ventricular tissues of L-NAME-treated rats. Effects of ET-1 on CaR expression and cell function (load free cell shortening) were analyzed in adult rat ventricular cardiomyocytes. siRNA directed against CaR and RAMP-1 was used to investigate a causal relationship. PD142893 and BQ788 were used to dissect the contribution of ETB1, ETB2, and ETA receptors. Non-specific NO synthase inhibition with L-Nitro arginine methyl ester (L-NAME) caused a cardiac upregulation of ETB receptors and CaR suggesting a paracrine effect of ET-1 on cardiomyocytes. Indeed, ET-1 induced the expression of CaR in cultured cardiomyocytes. Under these conditions, cardiomyocytes increased cell size (hypertrophy) but maintained normal function. Inhibition of ETA and ETB1 receptors led to ET-1-dependent reduction in cell shortening and attenuated up-regulation of CaR. Down-regulation of RAMP-1 reduced CaR responsiveness. In conclusion, ET-1 causes an adaptive type of hypertrophy by up-regulation of CaR in cardiomyocytes via ETA and/or ETB1 receptors. J. Cell. Physiol. 232: 2508–2518, 2017. © 2016 Wiley Periodicals, Inc. |
|
dc.relation.ispartofseries |
Journal of Cellular Physiology |
|
dc.title |
The Role of Calcium-Sensing Receptors in Endothelin-1-Dependent Effects on Adult Rat Ventricular Cardiomyocytes: Possible Contribution to Adaptive Myocardial Hypertrophy |
|
dc.type |
Article |
|
dc.relation.ispartofseries-issue |
9 |
|
dc.relation.ispartofseries-volume |
232 |
|
dc.collection |
Публикации сотрудников КФУ |
|
dc.relation.startpage |
2508 |
|
dc.source.id |
SCOPUS00219541-2017-232-9-SID85017588836 |
|