Показать сокращенную информацию
dc.contributor.author | Dyukova E. | |
dc.contributor.author | Schreckenberg R. | |
dc.contributor.author | Arens C. | |
dc.contributor.author | Sitdikova G. | |
dc.contributor.author | Schlüter K. | |
dc.date.accessioned | 2018-09-19T20:14:12Z | |
dc.date.available | 2018-09-19T20:14:12Z | |
dc.date.issued | 2017 | |
dc.identifier.issn | 0021-9541 | |
dc.identifier.uri | https://dspace.kpfu.ru/xmlui/handle/net/142721 | |
dc.description.abstract | © 2016 Wiley Periodicals, Inc.Nitric oxide (NO)-deficiency as it occurs during endothelial dysfunction activates the endothelin-1 (ET-1) system and increases the expression of receptor activity modifying protein (RAMP)-1 that acts as a chaperon for calcium-sensing receptors (CaR) that have recently been identified to improve cardiac function. Here, we hypothesized that ET-1 increases the cardiac expression of CaR and thereby induces an adaptive type of hypertrophy. Expressions of RAMP-1, endothelin receptors, and CaR were analyzed by RT-PCR in left ventricular tissues of L-NAME-treated rats. Effects of ET-1 on CaR expression and cell function (load free cell shortening) were analyzed in adult rat ventricular cardiomyocytes. siRNA directed against CaR and RAMP-1 was used to investigate a causal relationship. PD142893 and BQ788 were used to dissect the contribution of ETB1, ETB2, and ETA receptors. Non-specific NO synthase inhibition with L-Nitro arginine methyl ester (L-NAME) caused a cardiac upregulation of ETB receptors and CaR suggesting a paracrine effect of ET-1 on cardiomyocytes. Indeed, ET-1 induced the expression of CaR in cultured cardiomyocytes. Under these conditions, cardiomyocytes increased cell size (hypertrophy) but maintained normal function. Inhibition of ETA and ETB1 receptors led to ET-1-dependent reduction in cell shortening and attenuated up-regulation of CaR. Down-regulation of RAMP-1 reduced CaR responsiveness. In conclusion, ET-1 causes an adaptive type of hypertrophy by up-regulation of CaR in cardiomyocytes via ETA and/or ETB1 receptors. J. Cell. Physiol. 232: 2508–2518, 2017. © 2016 Wiley Periodicals, Inc. | |
dc.relation.ispartofseries | Journal of Cellular Physiology | |
dc.title | The Role of Calcium-Sensing Receptors in Endothelin-1-Dependent Effects on Adult Rat Ventricular Cardiomyocytes: Possible Contribution to Adaptive Myocardial Hypertrophy | |
dc.type | Article | |
dc.relation.ispartofseries-issue | 9 | |
dc.relation.ispartofseries-volume | 232 | |
dc.collection | Публикации сотрудников КФУ | |
dc.relation.startpage | 2508 | |
dc.source.id | SCOPUS00219541-2017-232-9-SID85017588836 |