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Expression of DNA-Encoded Antidote to Organophosphorus Toxins in the Methylotrophic Yeast Pichia Pastoris

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dc.contributor.author Terekhov S.
dc.contributor.author Bobik T.
dc.contributor.author Mokrushina Y.
dc.contributor.author Stepanova A.
dc.contributor.author Aleksandrova N.
dc.contributor.author Smirnov I.
dc.contributor.author Belogurov A.
dc.contributor.author Ponomarenko N.
dc.contributor.author Gabibov A.
dc.date.accessioned 2018-09-19T20:04:08Z
dc.date.available 2018-09-19T20:04:08Z
dc.date.issued 2016
dc.identifier.issn 0003-6838
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/142510
dc.description.abstract © 2016, Pleiades Publishing, Inc.A platform for the cloning and expression of active human butyrylcholinesterase (BuChE) in the yeast Pichia рastoris is first presented. Genetic constructs for BuChE gene expression, separately and in conjunction with a proline-rich peptide called proline-rich attachment domain (PRAD), are based on the vector pPICZαA. It is shown that the highest level of production is achieved in the expression of a BuChE gene without PRAD pPICZαA. It is found that one can obtain up to 125 mg of active enzyme from 1 L of culture medium at an optimal pH environment (pH 7.6), an optical seed culture density of 3 o.u., and an optimum methanol addition mode of (0.5% methanol in the first day and 0.2% thereafter from the second day).
dc.relation.ispartofseries Applied Biochemistry and Microbiology
dc.subject biological antidotes
dc.subject butyrylcholinesterase
dc.subject organophosphorus toxins
dc.subject Pichia рastoris
dc.title Expression of DNA-Encoded Antidote to Organophosphorus Toxins in the Methylotrophic Yeast Pichia Pastoris
dc.type Article
dc.relation.ispartofseries-issue 2
dc.relation.ispartofseries-volume 52
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 162
dc.source.id SCOPUS00036838-2016-52-2-SID84962145194


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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