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Excessive labeling technique provides a highly sensitive fluorescent probe for real-time monitoring of biodegradation of biopolymer pharmaceuticals in vivo

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dc.contributor.author Terekhov S.
dc.contributor.author Smirnov I.
dc.contributor.author Shamborant O.
dc.contributor.author Zenkova M.
dc.contributor.author Chernolovskaya E.
dc.contributor.author Gladkikh D.
dc.contributor.author Murashev A.
dc.contributor.author Dyachenko I.
dc.contributor.author Knorre V.
dc.contributor.author Belogurov A.
dc.contributor.author Ponomarenko N.
dc.contributor.author Deyev S.
dc.contributor.author Vlasov V.
dc.contributor.author Gabibov A.
dc.date.accessioned 2018-09-18T20:48:13Z
dc.date.available 2018-09-18T20:48:13Z
dc.date.issued 2014
dc.identifier.issn 2075-8251
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/142327
dc.description.abstract © 2014 Park-media, Ltd. Recombinant proteins represent a large sector of the biopharma market. Determination of the main elimination pathways raises the opportunities to significantly increase their half-lives in vivo. However, evaluation of biodegradation of pharmaceutical biopolymers performed in the course of pre-clinical studies is frequently complicated. Noninvasive pharmacokinetic and biodistribution studies in living organism are possible using proteins conjugated with near-infrared dyes. In the present study we designed a highly efficient probe based on fluorescent dye self-quenching for monitoring of in vivo biodegradation of recombinant human butyrylcholinesterase. The maximum enhancement of integral fluorescence in response to degradation of an intravenously administered enzyme was observed 6 h after injection. Importantly, excessive butyrylcholinesterase labeling with fluorescent dye results in significant changes in the pharmacokinetic properties of the obtained conjugate. This fact must be taken into consideration during future pharmacokinetic studies using in vivo bioimaging.
dc.relation.ispartofseries Acta Naturae
dc.subject Biodegradation
dc.subject Butyrylcholinesterase
dc.subject Fluorescent probe
dc.subject In vivo bioimaging
dc.subject Pharmacokinetics
dc.subject Proteolysis
dc.subject Self-quenching
dc.title Excessive labeling technique provides a highly sensitive fluorescent probe for real-time monitoring of biodegradation of biopolymer pharmaceuticals in vivo
dc.type Article
dc.relation.ispartofseries-issue 23
dc.relation.ispartofseries-volume 6
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 54
dc.source.id SCOPUS20758251-2014-6-23-SID84920145051


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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