Optimization of cultivation media for heterologous gene expression of adamalysin-like melalloendopeptidase Bacillus pumilus

dc.contributor.author	Rudakova N.
dc.contributor.author	Balaban N.
dc.contributor.author	Mardanova A.
dc.contributor.author	Chastukhina I.
dc.contributor.author	Sharipova M.
dc.date.accessioned	2018-09-18T20:37:09Z
dc.date.available	2018-09-18T20:37:09Z
dc.date.issued	2015
dc.identifier.uri	https://dspace.kpfu.ru/xmlui/handle/net/141760
dc.description.abstract	© 2015 Rudakova et al. The paper is dedicated to selecting main components of the nutrient medium and additional sources of nutrients for the maximum production of adamalysin-like metalloproteinase Bacillus pumilus. For convenience, the gene of metalloproteinase MprBp was cloned in protein-deficient strain B. subtilis BG2036. During the study, optimal concentration of peptone, inorganic phosphate, and casein was selected. Besides, it was shown that the enzyme production is stimulated by kasamino acids, leucine, alanine, asparagine, glutamine, as well as calcium and magnesium ions.
dc.subject	Adamalysine-like
dc.subject	Bacillus pumilus
dc.subject	Culture fluid
dc.subject	Metalloproteinase
dc.subject	Optimization
dc.subject	Productivity
dc.title	Optimization of cultivation media for heterologous gene expression of adamalysin-like melalloendopeptidase Bacillus pumilus
dc.type	Article
dc.relation.ispartofseries-issue	2
dc.relation.ispartofseries-volume	7
dc.collection	Публикации сотрудников КФУ
dc.source.id	SCOPUS-2015-7-2-SID84928709517

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Публикации сотрудников КФУ Scopus [24551]
Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.