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Optimization of cultivation media for heterologous gene expression of adamalysin-like melalloendopeptidase Bacillus pumilus

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dc.contributor.author Rudakova N.
dc.contributor.author Balaban N.
dc.contributor.author Mardanova A.
dc.contributor.author Chastukhina I.
dc.contributor.author Sharipova M.
dc.date.accessioned 2018-09-18T20:37:09Z
dc.date.available 2018-09-18T20:37:09Z
dc.date.issued 2015
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/141760
dc.description.abstract © 2015 Rudakova et al. The paper is dedicated to selecting main components of the nutrient medium and additional sources of nutrients for the maximum production of adamalysin-like metalloproteinase Bacillus pumilus. For convenience, the gene of metalloproteinase MprBp was cloned in protein-deficient strain B. subtilis BG2036. During the study, optimal concentration of peptone, inorganic phosphate, and casein was selected. Besides, it was shown that the enzyme production is stimulated by kasamino acids, leucine, alanine, asparagine, glutamine, as well as calcium and magnesium ions.
dc.subject Adamalysine-like
dc.subject Bacillus pumilus
dc.subject Culture fluid
dc.subject Metalloproteinase
dc.subject Optimization
dc.subject Productivity
dc.title Optimization of cultivation media for heterologous gene expression of adamalysin-like melalloendopeptidase Bacillus pumilus
dc.type Article
dc.relation.ispartofseries-issue 2
dc.relation.ispartofseries-volume 7
dc.collection Публикации сотрудников КФУ
dc.source.id SCOPUS-2015-7-2-SID84928709517


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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