Heterologous expression of Bacillus ginsengihumi phytase gene

dc.contributor.author	Akhmetova A.
dc.contributor.author	Suleimanova A.
dc.contributor.author	Toymentseva A.
dc.contributor.author	Balaban N.
dc.contributor.author	Iljukhina D.
dc.contributor.author	Sharipova M.
dc.date.accessioned	2018-09-18T20:36:54Z
dc.date.available	2018-09-18T20:36:54Z
dc.date.issued	2015
dc.identifier.uri	https://dspace.kpfu.ru/xmlui/handle/net/141713
dc.description.abstract	Phytate is the most abundant reservoir of phosphorus, which can potentially be used by microorganisms, plants and animals. Microbial phytases, capable of hydrolysing insoluble phytates, can be used as animal feed additives. In the present study, we isolated new phytase-producing bacteria - Bacillus ginsengihumi M2.11, which was identified using Microbial Identification System and 16S rRNA sequencing. We first cloned and sequenced the 1149 bp open reading frame encoding Bacillus ginsengihumi M2.11 phytase into the expression vector pET-46 Ek/LIC by the LIC-cloning method (cloning without ligase). The recombinant E.coli strain expressing Bacillus ginsengihumi M2.11 phytase was obtained. The molecular weight of phytase was 41 kDa.
dc.subject	Bacillus
dc.subject	Cloning
dc.subject	Expression
dc.subject	Phytase
dc.title	Heterologous expression of Bacillus ginsengihumi phytase gene
dc.type	Article
dc.relation.ispartofseries-issue	4
dc.relation.ispartofseries-volume	6
dc.collection	Публикации сотрудников КФУ
dc.relation.startpage	117
dc.source.id	SCOPUS-2015-6-4-SID84937918204

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Публикации сотрудников КФУ Scopus [24551]
Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.