Kazan Federal University Digital Repository

Analysis of the organic solvent effect on the hydration and structure of alpha-chymotrypsin by ftir spectroscopy

Show simple item record

dc.contributor.author Sirotkin V.
dc.date.accessioned 2018-09-18T20:36:23Z
dc.date.available 2018-09-18T20:36:23Z
dc.date.issued 2014
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/141627
dc.description.abstract © 2014 by Nova Science Publishers, Inc. All rights reserved. The effect of acetonitrile (AN) on the hydration and structure of bovine pancreatic α-chymotrypsin (CT) was characterized using FTIR spectroscopy at 25°C. The obtained results show that the hydration and structure of a-chymotrypsin depend markedly on how enzyme has been hydrated - whether in the presence or in the absence of organic solvent. Acetonitrile decreases markedly the water content at a given water activity (aw). This behavior suggests that the suppression in the uptake of water is due to a competition for water-binding sites on α-chymotrypsin by acetonitrile. Changes in the structure of CT were determined from infrared spectra by analyzing the structure of amide I band. It was found that, at low water activity, the protein-protein contacts in the dried enzyme largely govern its thermodynamic, structural, and functional properties. At high water activity, the chymotrypsin state is determined by the enzyme-organic solvent and enzyme-water interactions resulting in the enzyme denaturation. The interplay of these two factors is responsible for the observed complex shape of the enzyme storage stability - aw curves, with an extremum at intermediate water activity values.
dc.subject Acetonitrile
dc.subject Biocatalysis in organic solvents
dc.subject Bovine pancreatic α-chymotrypsin
dc.subject Enzyme hydration and structure
dc.subject Fourier transform infrared spectroscopy (FTIR spectroscopy)
dc.title Analysis of the organic solvent effect on the hydration and structure of alpha-chymotrypsin by ftir spectroscopy
dc.type Chapter
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 107
dc.source.id SCOPUS-2014-SID84955149915


Files in this item

This item appears in the following Collection(s)

  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

Show simple item record

Search DSpace


Advanced Search

Browse

My Account

Statistics