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A study of the heat capacity of ribonuclease a - water mixtures

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dc.contributor.author Sirotkin V.
dc.date.accessioned 2018-09-18T20:36:22Z
dc.date.available 2018-09-18T20:36:22Z
dc.date.issued 2014
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/141625
dc.description.abstract © 2014 by Nova Science Publishers, Inc. All rights reserved. Excess heat capacities of the binary system of bovine pancreatic ribonuclease A (RNase A) with water were obtained as a function of composition at 25°C. Differential scanning calorimetry was applied to study hydration dependencies of the excess thermodynamic functions. A major focus of this study aims to show how these thermodynamic quantities correlate with coverage of the protein by the water molecules. The excess partial quantities are found to be sensitive to changes in the water and protein states. At the lowest water weight fractions (w1), the changes of the excess functions can mainly be attributed to water addition. A transition from the glassy to the flexible state of the protein is accompanied by significant changes in the excess partial quantities of water and lysozyme. This transition appears at w1 > of 0.05 when charged groups of the protein are covered. Excess partial quantities reach their fully hydrated values at w1 > 0.5 when coverage of both polar and weakly interacting surface elements is complete. At the highest water contents, water addition has no significant effect on the excess quantities. At w1 > 0.5, changes in the excess functions can solely be attributed to changes in the state of the protein.
dc.subject Biocalorimetry
dc.subject Excess partial quantities
dc.subject Heat capacity
dc.subject Hydration of proteins (biomacromolecules)
dc.title A study of the heat capacity of ribonuclease a - water mixtures
dc.type Chapter
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 71
dc.source.id SCOPUS-2014-SID84955099874


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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