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Some features of hydrolysis of the hybrid B-Z-form dna by serratia marcescens nuclease

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dc.contributor.author Filimonova M.
dc.contributor.author Gubskaya V.
dc.contributor.author Nuretdinov I.
dc.date.accessioned 2018-09-18T20:35:56Z
dc.date.available 2018-09-18T20:35:56Z
dc.date.issued 2014
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/141543
dc.description.abstract Highly polymerized herring testis DNA of the random nucleotide sequence was used as a model of natural substrate to study some features of hydrolysis of the hybrid B-Z form with Serratia marcescens nuclease. The hybrid B-Z-form was formed upon addition of 1.15. M MgSO4 and 0.421 mM Co(NH3)6Cl3. The DNA transition from the right handed B-form to the hybrid B-Z-form caused a decrease in Vmax of DNA cleavage with the nuclease. The diminishing Vmax was consistent with diminishing values of Km and Kcat. The binding of Mg2+ or Co(NH3)6 3+ to highly polymerized DNA caused correspondingly about 80-or 7-fold decrease in Km and more than 1600 or 600 decrease in Kcat compared with that of Mg-DNA complex of B-form. © 2014 Science Publication.
dc.subject Hybrid B-Z-form DNA
dc.subject Serratia marcescens nuclease
dc.subject Sma Nuc
dc.title Some features of hydrolysis of the hybrid B-Z-form dna by serratia marcescens nuclease
dc.type Article
dc.relation.ispartofseries-issue 3
dc.relation.ispartofseries-volume 14
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 179
dc.source.id SCOPUS-2014-14-3-SID84905191722


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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