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Variability in the relative human DNA content during metagenomic analysis of gut microbiota

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dc.contributor.author Kostryukova E.
dc.contributor.author Karpova I.
dc.contributor.author Larin A.
dc.contributor.author Popenko A.
dc.contributor.author Tyaht A.
dc.contributor.author Ilina E.
dc.date.accessioned 2018-09-18T20:33:33Z
dc.date.available 2018-09-18T20:33:33Z
dc.date.issued 2015
dc.identifier.issn 1990-7508
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/141137
dc.description.abstract © 2015, Pleiades Publishing, Ltd. The comparative study of seven different methods for extraction of total DNA from human feces has been carried out. All these methods are recommended in protocols for metagenomic analysis of human gut microbiota. The relative abundance of human DNA in such samples registered by shotgun sequencing on a SOLiD 4 genetic analyzer has been investigated. It was shown that either initial amount of feces or a method applied for total DNA extraction insignificantly influenced the final relative human DNA proportion, which did not exceed 1% in healthy people. Invariance of this parameter allows to consider the increased proportion of human DNA in metagenomic samples as a potential marker of inflammatory bowel diseases.
dc.relation.ispartofseries Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry
dc.subject DNA extraction from feces
dc.subject metagenomic analysis
dc.subject shotgun sequencing
dc.title Variability in the relative human DNA content during metagenomic analysis of gut microbiota
dc.type Article
dc.relation.ispartofseries-issue 3
dc.relation.ispartofseries-volume 9
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 290
dc.source.id SCOPUS19907508-2015-9-3-SID84940190657


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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