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Internalization of Bacillus intermedius ribonuclease (BINASE) induces human alveolar adenocarcinoma cell death

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dc.contributor.author Cabrera-Fuentes H.
dc.contributor.author Aslam M.
dc.contributor.author Saffarzadeh M.
dc.contributor.author Kolpakov A.
dc.contributor.author Zelenikhin P.
dc.contributor.author Preissner K.
dc.contributor.author Ilinskaya O.
dc.date.accessioned 2018-09-18T20:32:14Z
dc.date.available 2018-09-18T20:32:14Z
dc.date.issued 2013
dc.identifier.issn 0041-0101
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/140904
dc.description.abstract Ribonuclease (RNase) treatment represents a novel mechanism based approach to anticancer therapy as an alternative to the DNA damaging drugs commonly used in clinical practice. Apart from their ribonucleolytic activity, cytotoxic effects have attracted a considerable attention to RNases because of their potential as selective agents for treatment of certain malignancies. Among these enzymes, Binase, an RNase from Bacillus intermedius, has shown promising results. Here, we have found that binase selectively attacked human A549 alveolar adenocarcinoma cells to trigger an apoptotic response, whereas normal lung epithelial cells LEK were not affected by the ribonuclease. The tumor transformation led to the modification of certain cellular characteristics causing cell sensitivity to binase. Although a general mode for RNases cytotoxicity includes their penetration into the cell, translocation to the cytosol and degradation of ribonucleic acid, many aspects of this process have not been fully elucidated. Our data revealed the following time-dependent changes induced by binase in A549 cells: (a) fast permanent internalization of the enzyme during the first hours of treatment; (b) temporary increase in cellular permeability for macromolecules during the 4-6hof treatment; (c) apoptotic alterations in population after 24hand (d) DNA fragmentation and cell death after 72hof treatment with binase. Elucidation of these molecular strategies used by this promising toxin provides us essential information for the development of new anticancer drugs. © 2013 Elsevier Ltd.
dc.relation.ispartofseries Toxicon
dc.subject Apoptosis
dc.subject Binase
dc.subject Cytotoxic RNase
dc.subject DSB
dc.subject Human alveolar adenocarcinoma cells
dc.subject Internalization
dc.subject Permeability
dc.title Internalization of Bacillus intermedius ribonuclease (BINASE) induces human alveolar adenocarcinoma cell death
dc.type Article
dc.relation.ispartofseries-volume 69
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 219
dc.source.id SCOPUS00410101-2013-69-SID84878478484


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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