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Acetylcholinesterase biosensor for inhibitor measurements based on glassy carbon electrode modified with carbon black and pillar[5]arene

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dc.contributor.author Shamagsumova R.
dc.contributor.author Shurpik D.
dc.contributor.author Padnya P.
dc.contributor.author Stoikov I.
dc.contributor.author Evtugyn G.
dc.date.accessioned 2018-09-18T20:31:56Z
dc.date.available 2018-09-18T20:31:56Z
dc.date.issued 2015
dc.identifier.issn 0039-9140
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/140848
dc.description.abstract © 2015 Elsevier B.V. Abstract New acetylcholinesterase (AChE) biosensor based on unsubstituted pillar[5]arene (P[5]A) as electron mediator was developed and successfully used for highly sensitive detection of organophosphate and carbamate pesticides. The AChE from electric eel was immobilized by carbodiimide binding on carbon black (CB) placed on glassy carbon electrode. The working potential of 200 mV was obtained in chronoamperometric mode with the measurement time of 180 s providing best inter-biosensors precision of the results. The AChE biosensor developed made it possible to detect 1×10<sup>-11</sup>-1×10<sup>-6</sup> M of malaoxon, 1×10<sup>-</sup><sup>8</sup>-7×10<sup>-6</sup> M of methyl-paraoxon, 1×10<sup>-10</sup>-2×10<sup>-6</sup> M of carbofuran and 7×10<sup>-9</sup>-1×10<sup>-5</sup> M of aldicarb with 10 min incubation. The limits of detection were 4×10<sup>-12</sup>, 5×10<sup>-9</sup>, 2×10<sup>-11</sup> and 6×10<sup>-10</sup> M, respectively. The AChE biosensor was tested in the analysis of pesticide residuals in spiked samples of peanut and beetroot. The protecting effect of P[5]A derivative bearing quaternary ammonia groups on malaoxon inhibition was shown.
dc.relation.ispartofseries Talanta
dc.subject Acetylcholinesterase biosensor
dc.subject Inhibitor determination
dc.subject Irreversible inhibition
dc.subject Pillar[5]arene
dc.subject Protective effect
dc.title Acetylcholinesterase biosensor for inhibitor measurements based on glassy carbon electrode modified with carbon black and pillar[5]arene
dc.type Article
dc.relation.ispartofseries-volume 144
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 559
dc.source.id SCOPUS00399140-2015-144-SID84938546318


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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