dc.contributor.author |
Starostina I. |
|
dc.contributor.author |
Solovyeva V. |
|
dc.contributor.author |
Shevchenko K. |
|
dc.contributor.author |
Deev R. |
|
dc.contributor.author |
Isaev A. |
|
dc.contributor.author |
Rizvanov A. |
|
dc.date.accessioned |
2018-09-18T20:30:31Z |
|
dc.date.available |
2018-09-18T20:30:31Z |
|
dc.date.issued |
2012 |
|
dc.identifier.issn |
1815-445X |
|
dc.identifier.uri |
https://dspace.kpfu.ru/xmlui/handle/net/140614 |
|
dc.description.abstract |
Dysferlinopathies belong to neuromuscular diseases associated with aberrant expression and/or function of dysferlin protein in skeletal muscle, which is caused by mutations in the dysf (dystrophy-associated fer-1-like, DYSF) gene. Because of the large size of the codon-optimized dysf coding region (6243 bp), adenoviral vectors are suitable for the creation of genetic constructs, which are capable of delivering a large amount of recombinant genetic information into both dividing and non-dividing cells, as well as provide a high level of transgene expression. We generated a recombinant adenovirus serotype 5 encoding a codon-optimized gene for human dysferlin (Ad5-Dysf) and analysed recombinant protein expression in vitro in HEK-293T cell line. |
|
dc.relation.ispartofseries |
Cellular Transplantation and Tissue Engineering |
|
dc.subject |
Codon optimization |
|
dc.subject |
Dysferlin |
|
dc.subject |
Gene therapy |
|
dc.subject |
Recombinant adenovirus |
|
dc.title |
Formation of the recombinant adenovirus encoding codon-optimized dysferlin gene and analysis of the recombinant protein expression in cell culture in vitro |
|
dc.type |
Article |
|
dc.relation.ispartofseries-issue |
3 |
|
dc.relation.ispartofseries-volume |
7 |
|
dc.collection |
Публикации сотрудников КФУ |
|
dc.relation.startpage |
25 |
|
dc.source.id |
SCOPUS1815445X-2012-7-3-SID84871840167 |
|