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dc.contributor.author | Starostina I. | |
dc.contributor.author | Solovyeva V. | |
dc.contributor.author | Shevchenko K. | |
dc.contributor.author | Deev R. | |
dc.contributor.author | Isaev A. | |
dc.contributor.author | Rizvanov A. | |
dc.date.accessioned | 2018-09-18T20:30:31Z | |
dc.date.available | 2018-09-18T20:30:31Z | |
dc.date.issued | 2012 | |
dc.identifier.issn | 1815-445X | |
dc.identifier.uri | https://dspace.kpfu.ru/xmlui/handle/net/140614 | |
dc.description.abstract | Dysferlinopathies belong to neuromuscular diseases associated with aberrant expression and/or function of dysferlin protein in skeletal muscle, which is caused by mutations in the dysf (dystrophy-associated fer-1-like, DYSF) gene. Because of the large size of the codon-optimized dysf coding region (6243 bp), adenoviral vectors are suitable for the creation of genetic constructs, which are capable of delivering a large amount of recombinant genetic information into both dividing and non-dividing cells, as well as provide a high level of transgene expression. We generated a recombinant adenovirus serotype 5 encoding a codon-optimized gene for human dysferlin (Ad5-Dysf) and analysed recombinant protein expression in vitro in HEK-293T cell line. | |
dc.relation.ispartofseries | Cellular Transplantation and Tissue Engineering | |
dc.subject | Codon optimization | |
dc.subject | Dysferlin | |
dc.subject | Gene therapy | |
dc.subject | Recombinant adenovirus | |
dc.title | Formation of the recombinant adenovirus encoding codon-optimized dysferlin gene and analysis of the recombinant protein expression in cell culture in vitro | |
dc.type | Article | |
dc.relation.ispartofseries-issue | 3 | |
dc.relation.ispartofseries-volume | 7 | |
dc.collection | Публикации сотрудников КФУ | |
dc.relation.startpage | 25 | |
dc.source.id | SCOPUS1815445X-2012-7-3-SID84871840167 |