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Influence of chronic food deprivation on structure-function relationship of juvenile rat fast muscles

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dc.contributor.author Ruiz-Rosado A.
dc.contributor.author Cabrera-Fuentes H.
dc.contributor.author González-Calixto C.
dc.contributor.author González-López L.
dc.contributor.author Cázares-Raga F.
dc.contributor.author Segura-Alegría B.
dc.contributor.author Lochnit G.
dc.contributor.author De La Cruz Hernández-Hernández F.
dc.contributor.author Preissner K.
dc.contributor.author Jiménez-Estrada I.
dc.date.accessioned 2018-09-18T20:28:53Z
dc.date.available 2018-09-18T20:28:53Z
dc.date.issued 2013
dc.identifier.issn 0142-4319
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/140343
dc.description.abstract In the present study, we analyze the influence of chronic undernutrition on protein expression, muscle fiber type composition, and fatigue resistance of the fast extensor digitorum longus (EDL) muscle of male juvenile rats (45 ± 3 days of life; n = 25 and 31 rats for control and undernourished groups, respectively). Using 2D gel electrophoresis and mass spectrometry, we identified in undernourished muscles 12 proteins up-regulated (8 proteins of the electron transport chain and the glycolytic pathway, 2 cross-bridge proteins, chaperone and signaling proteins that are related to the stress response). In contrast, one down-regulated protein related to the fast muscle contractile system and two other proteins with no changes in expression were used as charge controls. By means of COX and alkaline ATPase histochemical techniques and low-frequency fatigue protocols we determined that undernourished muscles showed a larger proportion (15 % increase) of Type IIa/IId fibers (oxidative- glycolytic) at the expense of Type IIb (glycolytic) fibers (15.5 % decrease) and increased fatigue resistance (55.3 %). In addition, all fiber types showed a significant reduction in their cross-sectional area (slow: 64.4 %; intermediate: 63.9 % and fast: 61.2 %). These results indicate that undernourished EDL muscles exhibit an increased expression of energy metabolic and myofibrillar proteins which are associated with the predominance of oxidative and Type IIa/IId fibers and to a higher resistance to fatigue. We propose that such alterations may act as protective and/or adaptive mechanisms that counterbalance the effect of chronic undernourishment. © 2013 Springer Science+Business Media Dordrecht.
dc.relation.ispartofseries Journal of Muscle Research and Cell Motility
dc.subject EDL muscle
dc.subject Glycolytic fibers
dc.subject Muscle fatigue
dc.subject Oxidative fibers
dc.subject Undernutrition
dc.title Influence of chronic food deprivation on structure-function relationship of juvenile rat fast muscles
dc.type Article
dc.relation.ispartofseries-issue 5-6
dc.relation.ispartofseries-volume 34
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 357
dc.source.id SCOPUS01424319-2013-34-56-SID84890118770


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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