dc.contributor.author |
Filimonova M. |
|
dc.contributor.author |
Gubskaya V. |
|
dc.contributor.author |
Davidov R. |
|
dc.contributor.author |
Garusov A. |
|
dc.contributor.author |
Nuretdinov I. |
|
dc.date.accessioned |
2018-09-18T20:28:53Z |
|
dc.date.available |
2018-09-18T20:28:53Z |
|
dc.date.issued |
2008 |
|
dc.identifier.issn |
0141-8130 |
|
dc.identifier.uri |
https://dspace.kpfu.ru/xmlui/handle/net/140341 |
|
dc.description.abstract |
Highly polymerized herring testis DNA of the random nucleotide sequence has been studied in solution by circular dichroism and ultra-violet absorption spectrometry under various experimental conditions. At low temperature upon addition of 0.05 M NaCl or 1.15 M MgSO4 the DNA formed a helix that belonged to the B-family. As the temperature was increased a transition from the pure B- to the hybrid B-Z-form occurred in the presence of 1.15 M MgSO4. This transition occurred over a large range of temperatures and corresponded to a non-cooperative conformational change. A similar DNA transition was induced with 0.098 mM Co(NH3)6Cl3. However, in the presence of 5.3 M NaCl the DNA conformation was not similar to that observed in 1.15 M MgSO4 or 0.098 mM Co(NH3)6Cl3 independently on the environmental temperature. In 5.3 M NaCl the DNA is thought to undergo a transition from one to another right-handed conformation that could be intermediate partially dehydrated conformer arising on the first step in the sequential transition to the dehydration of the polynucleotide. Our results show that a realistic model of native DNA, bearing Z-tracts embedded in B-helixes, can be obtained upon binding of alkaline earth or transition metals. © 2008 Elsevier B.V. All rights reserved. |
|
dc.relation.ispartofseries |
International Journal of Biological Macromolecules |
|
dc.subject |
DNA conformation |
|
dc.subject |
Hybrid B-Z-form |
|
dc.subject |
Native DNA |
|
dc.title |
Metal binding induces conversion of B- to the hybrid B-Z-form in natural DNA |
|
dc.type |
Article |
|
dc.relation.ispartofseries-issue |
3 |
|
dc.relation.ispartofseries-volume |
43 |
|
dc.collection |
Публикации сотрудников КФУ |
|
dc.relation.startpage |
289 |
|
dc.source.id |
SCOPUS01418130-2008-43-3-SID49749114834 |
|