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dc.contributor.author | Zefirov A. | |
dc.contributor.author | Zakharov A. | |
dc.contributor.author | Mukhametzyanov R. | |
dc.contributor.author | Petrov A. | |
dc.contributor.author | Sitdikova G. | |
dc.date.accessioned | 2018-09-18T20:28:29Z | |
dc.date.available | 2018-09-18T20:28:29Z | |
dc.date.issued | 2009 | |
dc.identifier.issn | 0097-0549 | |
dc.identifier.uri | https://dspace.kpfu.ru/xmlui/handle/net/140269 | |
dc.description.abstract | Experiments on the mouse diaphragm muscle using intracellular microelectrode recordings and fluorescence microscopy were performed to study the dynamics of transmitter secretion and synaptic vesicle recycling processes (the exocytosis-endocytosis cycle) in motor nerve endings (NE) during prolonged rhythmic stimulation (20 impulses/sec). During stimulation, there were triphasic changes in the amplitude of endplate potentials (EPP): an initial rapid reduction, followed by prolonged (1-2 min) stabilization of amplitude, i.e., a plateau, and then a further slow decrease. Restoration of EPP amplitude after stimulation for 3 min occurred over a period of several seconds. Loading of synaptic vesicles with the fluorescent endocytic stain FM1-43 showed that rhythmic stimulation led to a gradual (over 5-6 min) decrease in NE fluorescence, demonstrating exocytosis of synaptic vesicles. Quantum analysis of the electrophysiological data and comparison of these data with results from fluorescence studies suggested that mouse NE have a high rate of endocytosis and reutilization of synaptic vesicles (the mean recycling time was about 50 sec), which may support the maintenance of reliable synaptic transmission during prolonged high-frequency activity. The sizes of the release-ready and recycling pools of synaptic vesicles were determined quantitatively. It is suggested that vesicle recycling in mouse NE occurs via a short, rapid pathway with incorporation into the recycling pool. Vesicles of the reserve pool are not used for transmitter secretion in the stimulation conditions used here. © 2009 Springer Science+Business Media, Inc. | |
dc.relation.ispartofseries | Neuroscience and Behavioral Physiology | |
dc.subject | Endocytosis | |
dc.subject | Endplate potentials | |
dc.subject | Exocytosis | |
dc.subject | FM1-43 | |
dc.subject | Mouse diaphragm motor nerve ending | |
dc.subject | Synaptic vesicle recycling | |
dc.title | The vesicle cycle in motor nerve endings of the mouse diaphragm | |
dc.type | Article | |
dc.relation.ispartofseries-issue | 3 | |
dc.relation.ispartofseries-volume | 39 | |
dc.collection | Публикации сотрудников КФУ | |
dc.relation.startpage | 245 | |
dc.source.id | SCOPUS00970549-2009-39-3-SID61549105614 |