dc.contributor.author |
Zefirov A. |
|
dc.contributor.author |
Zakharov A. |
|
dc.contributor.author |
Mukhametzyanov R. |
|
dc.contributor.author |
Petrov A. |
|
dc.contributor.author |
Sitdikova G. |
|
dc.date.accessioned |
2018-09-18T20:28:29Z |
|
dc.date.available |
2018-09-18T20:28:29Z |
|
dc.date.issued |
2009 |
|
dc.identifier.issn |
0097-0549 |
|
dc.identifier.uri |
https://dspace.kpfu.ru/xmlui/handle/net/140269 |
|
dc.description.abstract |
Experiments on the mouse diaphragm muscle using intracellular microelectrode recordings and fluorescence microscopy were performed to study the dynamics of transmitter secretion and synaptic vesicle recycling processes (the exocytosis-endocytosis cycle) in motor nerve endings (NE) during prolonged rhythmic stimulation (20 impulses/sec). During stimulation, there were triphasic changes in the amplitude of endplate potentials (EPP): an initial rapid reduction, followed by prolonged (1-2 min) stabilization of amplitude, i.e., a plateau, and then a further slow decrease. Restoration of EPP amplitude after stimulation for 3 min occurred over a period of several seconds. Loading of synaptic vesicles with the fluorescent endocytic stain FM1-43 showed that rhythmic stimulation led to a gradual (over 5-6 min) decrease in NE fluorescence, demonstrating exocytosis of synaptic vesicles. Quantum analysis of the electrophysiological data and comparison of these data with results from fluorescence studies suggested that mouse NE have a high rate of endocytosis and reutilization of synaptic vesicles (the mean recycling time was about 50 sec), which may support the maintenance of reliable synaptic transmission during prolonged high-frequency activity. The sizes of the release-ready and recycling pools of synaptic vesicles were determined quantitatively. It is suggested that vesicle recycling in mouse NE occurs via a short, rapid pathway with incorporation into the recycling pool. Vesicles of the reserve pool are not used for transmitter secretion in the stimulation conditions used here. © 2009 Springer Science+Business Media, Inc. |
|
dc.relation.ispartofseries |
Neuroscience and Behavioral Physiology |
|
dc.subject |
Endocytosis |
|
dc.subject |
Endplate potentials |
|
dc.subject |
Exocytosis |
|
dc.subject |
FM1-43 |
|
dc.subject |
Mouse diaphragm motor nerve ending |
|
dc.subject |
Synaptic vesicle recycling |
|
dc.title |
The vesicle cycle in motor nerve endings of the mouse diaphragm |
|
dc.type |
Article |
|
dc.relation.ispartofseries-issue |
3 |
|
dc.relation.ispartofseries-volume |
39 |
|
dc.collection |
Публикации сотрудников КФУ |
|
dc.relation.startpage |
245 |
|
dc.source.id |
SCOPUS00970549-2009-39-3-SID61549105614 |
|