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Interaction of secreted aspartic proteinase Candida albicans with ZnCl2: Complex formation and catalytic activity

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dc.contributor.author Kutyreva M.
dc.contributor.author Ulakhovich N.
dc.contributor.author Gataulina A.
dc.contributor.author Mukhametzyanova A.
dc.contributor.author Khaldeeva E.
dc.date.accessioned 2018-09-18T20:26:39Z
dc.date.available 2018-09-18T20:26:39Z
dc.date.issued 2013
dc.identifier.issn 1818-4952
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/139938
dc.description.abstract The aim of work is to estimate ion Zn(II) influence on catalytic activity of secreted aspartic proteinase Candida albicans (SAP2). System ZnCl2 - SAP2 showed complex formation. Composition of [ZnmSAP2n] complex was 1:1, the stability constant was 4.73±0.20. Affinity constants in system SAP2 - human serum albumin (HSA) - ZnCl2 were determined at Skatchard coordinates. The SAP2 was found to have a one section for linking with the substrate and one section for linking with modulator. The affinity constants in SAP2 - HSA system decreased in presence of ZnCl2. Complex forming of SAP2 - ZnCl2 system resulted in reduction of enzyme-substrate bonding. Proteolytic activity of SAP2 towards HSA at presence ZnCl2 as modulator was estimated. The inhibition effect in range 1×10-4 - 4×10-6 and 6×10-7 - 1×10-8 M was observed. The SAP2 activation effect catalyzed by ZnCl2 at 5×10-7 - 5×10-6 M concentrations were fixed for the first time. © IDOSI Publications, 2013.
dc.relation.ispartofseries World Applied Sciences Journal
dc.subject Enzyme activity
dc.subject Metal complex
dc.subject Proteinase Candida albicans
dc.subject Zinc ion
dc.title Interaction of secreted aspartic proteinase Candida albicans with ZnCl2: Complex formation and catalytic activity
dc.type Article
dc.relation.ispartofseries-issue 9
dc.relation.ispartofseries-volume 27
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 1225
dc.source.id SCOPUS18184952-2013-27-9-SID84890922868


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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