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dc.contributor.author | Minlebaev M. | |
dc.contributor.author | Valeeva G. | |
dc.contributor.author | Tcheremiskine V. | |
dc.contributor.author | Coustillier G. | |
dc.contributor.author | Khazipov R. | |
dc.date.accessioned | 2018-09-18T20:24:15Z | |
dc.date.available | 2018-09-18T20:24:15Z | |
dc.date.issued | 2013 | |
dc.identifier.issn | 1662-5102 | |
dc.identifier.uri | https://dspace.kpfu.ru/xmlui/handle/net/139527 | |
dc.description.abstract | We present a novel non-invasive technique to measure the polarity of GABAergic responses based on cell-attached recordings of currents activated by laser-uncaging of GABA. For these recordings, a patch pipette was filled with a solution containing RuBi-GABA, and GABA was released from this complex by a laser beam conducted to the tip of the patch pipette via an optic fiber. In cell-attached recordings from neocortical and hippocampal neurons in postnatal days P2-5 rat brain slices in vitro, we found that laser-uncaging of GABA activates integral cell-attached currents mediated by tens of GABA(A) channels. The initial response was inwardly directed, indicating a depolarizing response to GABA. The direction of the initial response was dependent on the pipette potential and analysis of its slope-voltage relationships revealed a depolarizing driving force of +11 mV for the currents through G. Current-voltage relationships of the currents evoked by Rubi-GABA uncaging using voltage-ramps at the peak of responses not only revealed a bumetanide-sensitive depolarizing reversal potential of the GABA(A) receptor mediated responses, but also showed a strong voltage-dependent hysteresis. Upon desensitization of the uncaged-GABA response, current-voltage relationships of the currents through single GABA(A) channels revealed depolarizing responses with the driving force values similar to those obtained for the initial response. Thus, cell-attached recordings of the responses evoked by local intrapipette GABA uncaging are suitable to assess the polarity of the GABA(A)-Rs mediated signals in small cell compartments. © 2013 Minlebaev, Valeeva, Tcheremiskine, Coustillier and Khazipov. | |
dc.relation.ispartofseries | Frontiers in Cellular Neuroscience | |
dc.subject | Cell-attach | |
dc.subject | GABA | |
dc.subject | Inhibitory postsynaptic potentials | |
dc.subject | Patch-clamp | |
dc.subject | Rubi-GABA | |
dc.subject | Uncaged GABA | |
dc.title | Cell-attached recordings of responses evoked by photorelease of GABA in the immature cortical neurons | |
dc.type | Article | |
dc.relation.ispartofseries-issue | MAY | |
dc.collection | Публикации сотрудников КФУ | |
dc.source.id | SCOPUS16625102-2013--SID84878105012 |